PMID- 18998531
OWN - NLM
STAT- MEDLINE
DCOM- 20090924
LR  - 20211203
IS  - 1000-3061 (Print)
IS  - 1000-3061 (Linking)
VI  - 24
IP  - 8
DP  - 2008 Aug
TI  - [Screening hv-S/TPK from TAC library of a Triticum aestivum-Haynaldia villosa 
      translocation line].
PG  - 1327-32
AB  - Hv-S/TPK gene, a resistance related gene to powdery mildew, was cloned by using 
      genechip, and its expression was upregulated after the inoculation of Blumeria 
      graminis to Haynaldia villosa. Using the specific primers of Hv-S/TPK to screen a 
      genomic TAC (Transformation-competent artificial chromosome) library of 
      translocation line 6VS/6AL, a positive TAC was screened. A 5-kb fragment 
      containing Hv-S/TPK was subcloned and identified. This 5160-bp fragment (GenBank 
      Accession No. EU153366) was determined by specific primer walking. The analysis 
      of Hv-S/TPK genomic sequence showed three introns and four extrons between start 
      code and stop code. In the promoter region of Hv-S/TPK, there were W-box and 
      OCS-like elements which were the elements related to disease resistance. In this 
      study, the positive TAC clone was used to as probe in situ hybridized to mitotic 
      metaphase chromosomes of translocation line. The result of fluorescence in situ 
      hybridization (FISH) indicated that the TAC clone containing Hv-S/TPK was from 
      Haynaldia villosa chromosome.
FAU - Sun, Yulei
AU  - Sun Y
AD  - National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 
      Agricultural University, Nanjing 210095, China.
FAU - Cao, Aizhong
AU  - Cao A
FAU - Yang, Xueming
AU  - Yang X
FAU - Wang, Xiaoyun
AU  - Wang X
FAU - Chen, Peidu
AU  - Chen P
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Base Sequence
MH  - *Chromosomes, Artificial
MH  - Cloning, Molecular
MH  - Genomic Library
MH  - Molecular Sequence Data
MH  - Plant Diseases/genetics
MH  - Poaceae/*genetics
MH  - Protein Serine-Threonine Kinases/*genetics
MH  - Serine/genetics
MH  - *Translocation, Genetic
MH  - Triticum/*genetics/virology
EDAT- 2008/11/13 09:00
MHDA- 2009/09/25 06:00
CRDT- 2008/11/13 09:00
PHST- 2008/11/13 09:00 [pubmed]
PHST- 2009/09/25 06:00 [medline]
PHST- 2008/11/13 09:00 [entrez]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2008 Aug;24(8):1327-32.