PMID- 19000142
OWN - NLM
STAT- MEDLINE
DCOM- 20090824
LR  - 20191210
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 72
IP  - 6
DP  - 2008 Dec
TI  - Development and clinical evaluation of a microarray for HLA-A and -DRB1 
      genotyping.
PG  - 568-77
LID - 10.1111/j.1399-0039.2008.01154.x [doi]
AB  - Microarray technology makes high-throughput genotyping possible by permitting the 
      simultaneous analysis of large sets of genes on a small reaction slide. Human 
      leukocyte antigen (HLA) loci showing high polymorphisms are suitable targets for 
      microarray. In this study, we developed a microarray kit with newly designed 
      oligonucleotide probes for the genotyping of HLA-A and -DRB1. In total, 42 probes 
      were designed to hybridize to polymorphic sites for HLA-A and 36 for HLA-DRB1. 
      Asymmetric polymerase chain reaction (PCR) using four primers was performed to 
      amplify exon 2 of HLA-DRB1, whereas symmetric PCR was performed to amplify both 
      exons 2 and 3 of HLA-A. Evaluation of performance using samples from 138 Koreans 
      disclosed consistent microarray results with all sequence-based typing at the 
      low-resolution level. Despite the occurrence of ambiguities in 35 HLA-A (25.4%) 
      and 5 HLA-DRB1 (3.6%) cases, correct genotypes were assigned with high certainty 
      by referring to allele distribution in Koreans. These data clearly indicate that 
      our newly developed microarray kit is optimal in determining correct genotypes at 
      the low-resolution level in Koreans.
FAU - Lee, K R
AU  - Lee KR
AD  - Biocore Co., Seoul, Korea.
FAU - Park, E
AU  - Park E
FAU - Moon, S H
AU  - Moon SH
FAU - Kim, J-M
AU  - Kim JM
FAU - Kwon, O-J
AU  - Kwon OJ
FAU - Kim, M H
AU  - Kim MH
FAU - Sohn, Y-H
AU  - Sohn YH
FAU - Ko, S-Y
AU  - Ko SY
FAU - Oh, H-B
AU  - Oh HB
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081018
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
SB  - IM
MH  - Genotype
MH  - HLA-A Antigens/*genetics
MH  - HLA-DR Antigens/*genetics
MH  - HLA-DRB1 Chains
MH  - Humans
MH  - Korea
MH  - Oligonucleotide Array Sequence Analysis/*methods
MH  - Population/*genetics
MH  - Sensitivity and Specificity
EDAT- 2008/11/13 09:00
MHDA- 2009/08/25 09:00
CRDT- 2008/11/13 09:00
PHST- 2008/11/13 09:00 [pubmed]
PHST- 2009/08/25 09:00 [medline]
PHST- 2008/11/13 09:00 [entrez]
AID - TAN1154 [pii]
AID - 10.1111/j.1399-0039.2008.01154.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2008 Dec;72(6):568-77. doi: 10.1111/j.1399-0039.2008.01154.x. 
      Epub 2008 Oct 18.