PMID- 1900156
OWN - NLM
STAT- MEDLINE
DCOM- 19910403
LR  - 20190623
IS  - 0006-2952 (Print)
IS  - 0006-2952 (Linking)
VI  - 41
IP  - 5
DP  - 1991 Mar 1
TI  - Inhibitory effects of two structurally related carbocyanine laser dyes on the 
      activity of bovine heart mitochondrial and Paracoccus denitrificans 
      NADH-ubiquinone reductase. Evidence for a rotenone-type mechanism.
PG  - 677-84
AB  - Two cationic, lipophilic laser dyes, 1,1',3,3,3',3'-hexamethylindodicarbocyanine 
      iodide (HIDC) and 1,1',3,3,3',3'-hexamethylindotricarbocyanine iodide (HITC), 
      inhibit bovine heart mitochondrial and Paracoccus denitrificans NADH oxidase 
      activities. The mitochondrial I50 values were 0.5 microM (HIDC) and 1.2 microM 
      (HITC), and the P. denitrificans I50 values 1.2 microM (HIDC) and 1.5 microM 
      (HITC). Neither succinate nor cytochrome oxidase (EC 1.9.3.1) activities were 
      inhibited significantly by either compound, localizing the site of inhibition to 
      the segment of each electron transport chain between NADH and ubiquinone. With 
      submitochrondrial particles (SMP), NADH-dependent reduction of menadione, 
      duroquinone and coenzyme Q1 was inhibited markedly (HIDC was the more potent 
      inhibitor). Using purified complex I, only NADH-dependent reduction of 
      duroquinone and coenzyme Q1 was inhibited markedly (HIDC was the more potent 
      inhibitor) and reduction of menadione was inhibited slightly. With P. 
      denitrificans membrane vesicles, NADH-dependent reduction of menadione, juglone, 
      and coenzyme Q1 was inhibited slightly and duroquinone reduction was inhibited 
      markedly. Membrane-dependent interactions appear to be involved, since the 
      compounds were more inhibitory with membrane preparations than with complex I. 
      The mechanism of inhibition (except for the HIDC effect on coenzyme Q1 reduction 
      with P. denitrificans) appeared to be through the interaction of dye with the 
      rotenone site on NADH-ubiquinone reductase (EC 1.6.99.3), since 
      rotenone-insensitive preparations of complex I and P. denitrificans membrane 
      vesicles were also insensitive to HIDC and HITC inhibition.
FAU - Anderson, W M
AU  - Anderson WM
AD  - Indiana University School of Medicine, Northwest Center for Medical Education, 
      Gary 46408.
FAU - Chambers, B B
AU  - Chambers BB
FAU - Wood, J M
AU  - Wood JM
FAU - Benninger, L
AU  - Benninger L
LA  - eng
GR  - HL 32829/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biochem Pharmacol
JT  - Biochemical pharmacology
JID - 0101032
RN  - 0 (Carbocyanines)
RN  - 0 (Fluorescent Dyes)
RN  - 03L9OT429T (Rotenone)
RN  - 16595-48-5 (1,1',3,3,3',3'-hexamethylindotricarbocyanine)
RN  - 48221-03-0 (1,1',3,3,3',3'-hexamethylindodicarbocyanine)
RN  - EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone))
RN  - EC 1.6.99.- (Quinone Reductases)
SB  - IM
MH  - Animals
MH  - Carbocyanines/*pharmacology
MH  - Cattle
MH  - Dose-Response Relationship, Drug
MH  - Electron Transport/drug effects
MH  - Fluorescent Dyes/*pharmacology
MH  - Mitochondria, Heart/*drug effects/enzymology
MH  - NAD(P)H Dehydrogenase (Quinone)
MH  - Paracoccus denitrificans/*drug effects/enzymology
MH  - Quinone Reductases/*metabolism
MH  - Rotenone/*pharmacology
EDAT- 1991/03/01 00:00
MHDA- 1991/03/01 00:01
CRDT- 1991/03/01 00:00
PHST- 1991/03/01 00:00 [pubmed]
PHST- 1991/03/01 00:01 [medline]
PHST- 1991/03/01 00:00 [entrez]
AID - 0006-2952(91)90066-E [pii]
AID - 10.1016/0006-2952(91)90066-e [doi]
PST - ppublish
SO  - Biochem Pharmacol. 1991 Mar 1;41(5):677-84. doi: 10.1016/0006-2952(91)90066-e.