PMID- 19002809
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 33
IP  - 1-3
DP  - 2000 Jul
TI  - Evaluation of stable and highly productive gene amplified CHO cell line based on 
      the location of amplified genes.
PG  - 37-46
LID - 10.1023/A:1008111328771 [doi]
AB  - In order to establish an easy and quick construction method for obtaining a 
      stable and highly productive gene-amplified recombinant Chinese Hamster Ovary 
      (CHO) cell line, variouskinds of stepwise methotrexate (MTX) selection were 
      carriedout. The specific growth and production rates of the cell were compared 
      with each other, and the distribution of the amplified gene location was 
      determined using fluorescence in situ hybridization (FISH). The specific growth 
      andproduction rates of the cell pool reached the highest levels under the 
      selection condition in which the stepwise increase in the MTX concentration was 
      most gradual; about 82% of amplified genes were observed near the telomeric 
      region. During long-term cultivation without MTX, the percentage ofamplified 
      genes near the telomeric region hardly changed, butthat of amplified genes at 
      other regions decreased. Based on these results, stable and highly productive 
      cell pools could be easily and quickly constructed and amplified and gradual 
      stepwise increase of the MTX concentration. In addition, the FISH technique was 
      powerful tool to evaluate highly productiveand stable gene-amplified cells based 
      on the chromosomal location of the amplified gene.
FAU - Yoshikawa, T
AU  - Yoshikawa T
AD  - Department of Biotechnology, Graduate School of Engineering, Osaka University, 
      2-1 Yamada-oka, Suita, Osaka, 565-0871, Japan.
FAU - Nakanishi, F
AU  - Nakanishi F
FAU - Itami, S
AU  - Itami S
FAU - Kameoka, D
AU  - Kameoka D
FAU - Omasa, T
AU  - Omasa T
FAU - Katakura, Y
AU  - Katakura Y
FAU - Kishimoto, M
AU  - Kishimoto M
FAU - Suga, K
AU  - Suga K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC3466717
EDAT- 2008/11/13 09:00
MHDA- 2008/11/13 09:01
PMCR- 2001/07/01
CRDT- 2008/11/13 09:00
PHST- 2008/11/13 09:00 [pubmed]
PHST- 2008/11/13 09:01 [medline]
PHST- 2008/11/13 09:00 [entrez]
PHST- 2001/07/01 00:00 [pmc-release]
AID - 265673 [pii]
AID - 10.1023/A:1008111328771 [doi]
PST - ppublish
SO  - Cytotechnology. 2000 Jul;33(1-3):37-46. doi: 10.1023/A:1008111328771.