PMID- 19002827
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20240505
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 33
IP  - 1-3
DP  - 2000 Jul
TI  - Receptor activator of NF-kappaB ligand induces the fusion of mononuclear 
      preosteoclasts into multinucleated osteoclasts.
PG  - 203-11
LID - 10.1023/A:1008198120670 [doi]
AB  - The osteoclasts are bone-resorbing multinucleatedcells formed by the fusion of 
      mononuclearpreosteoclasts (pOCs) of hematopoietic origin.Although receptor 
      activator of NF-kappaBligand (RANKL) has been shown to regulate 
      osteoclastdifferentiation and function, its effect on the fusionof pOCs into 
      multinucleated osteoclast-like cells(OCLs) has not been known. Using our fusion 
      assaysystem, that is not contaminated with multinucleatedcells (MNCs) and 
      osteoblastic cells, we determined theeffect of RANKL on the fusion of pOCs into 
      MNCs. WhenpOCs were cultured on the plates, most of pOCs diedand disappeared from 
      the plates within 24 h in theabsence of additives, but pOCs were fused to 
      MNCswithin 6 h in the presence of RANKL. RANKL-inducedMNCs showed typical 
      properties of OCL such astartrate-resistant acid phosphatase (TRAP) 
      activity,actin ring formation, and bone-resorbing activity. Thefusion of pOCs 
      into OCLs induced by osteoblastic cellsor RANKL was inhibited by OPG/OCIF, but 
      that inducedby IL-1beta was not. Both RANKL- andIL-1beta-induced OCL formation 
      from pOCs wasinhibited by ZLLL-H, a peptide inhibitor ofproteasome. These 
      findings indicate that RANKLsupports the survival of pOCs and induces the 
      fusionof pOCs into OCLs and suggest that NF-kappaBactivation is involved in these 
      processes induced byRANKL and IL-1beta.
FAU - Woo, J T
AU  - Woo JT
AD  - Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, 
      Midori-ku, Yokohama, 226-8501, Japan.
FAU - Kato, M
AU  - Kato M
FAU - Takami, M
AU  - Takami M
FAU - Nagai, K
AU  - Nagai K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC3466709
EDAT- 2008/11/13 09:00
MHDA- 2008/11/13 09:01
PMCR- 2001/07/01
CRDT- 2008/11/13 09:00
PHST- 2008/11/13 09:00 [pubmed]
PHST- 2008/11/13 09:01 [medline]
PHST- 2008/11/13 09:00 [entrez]
PHST- 2001/07/01 00:00 [pmc-release]
AID - 264429 [pii]
AID - 10.1023/A:1008198120670 [doi]
PST - ppublish
SO  - Cytotechnology. 2000 Jul;33(1-3):203-11. doi: 10.1023/A:1008198120670.