PMID- 19002922
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 37
IP  - 3
DP  - 2001 Nov
TI  - Development and improvement of a serum-free suspension process for the production 
      of recombinant adenoviral vectors using HEK293 cells.
PG  - 189-98
LID - 10.1023/A:1020555310558 [doi]
AB  - Human Embryonic Kidney 293 (HEK293) cells were adapted into a serum-free 
      suspension medium through steps of gradual serum weaning for the production of 
      adenoviral (AdV) gene therapy vectors. The presence of sodium heparin in the 
      medium formulation reduced cell clumping dramatically in suspension culture. The 
      adapted cells were ready to grow either in serum-containing medium as an attached 
      culture or in serum-free medium in suspension culture. A scalable production 
      process was developed in shake flasks and was then evaluated in stirred tank 
      bioreactors. This process includes a growth phase in batch-mode followed by a 
      production phase involving medium perfusion and supplementation. Fortification 
      with calcium chloride post viral inoculation resulted in an increase in virus 
      production by at least one fold. Addition of stimulating agents such as sodium 
      butyrate, N-acetyl-L-cysteine (NAC), dimethyl sulfoxide(DMSO), or ethyl alcohol 
      post infection was shown to further improve virus production in a dose-dependent 
      manner. The serum-free suspension process described here should be suitable for 
      the manufacturing of other E1-deleted AdV vectors and could potentially be used 
      for the production of recombinant proteins by HEK293 cells.
FAU - Tsao, Y S
AU  - Tsao YS
AD  - Biotechnology Development, Schering-Plough Research Institute, 1011 Morris 
      Avenue, 07083, Union, USA.
FAU - Condon, R
AU  - Condon R
FAU - Schaefer, E
AU  - Schaefer E
FAU - Lio, P
AU  - Lio P
FAU - Liu, Z
AU  - Liu Z
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC3449791
EDAT- 2008/11/13 09:00
MHDA- 2008/11/13 09:01
PMCR- 2002/11/01
CRDT- 2008/11/13 09:00
PHST- 2008/11/13 09:00 [pubmed]
PHST- 2008/11/13 09:01 [medline]
PHST- 2008/11/13 09:00 [entrez]
PHST- 2002/11/01 00:00 [pmc-release]
AID - 392361 [pii]
AID - 10.1023/A:1020555310558 [doi]
PST - ppublish
SO  - Cytotechnology. 2001 Nov;37(3):189-98. doi: 10.1023/A:1020555310558.