PMID- 19003058
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 49
IP  - 1
DP  - 2005 Sep
TI  - Enhancement of Sf9 Cells and Baculovirus Production Employing Grace's Medium 
      Supplemented with Milk Whey Ultrafiltrate.
PG  - 1-9
LID - 10.1007/s10616-005-4206-0 [doi]
AB  - Animal cells can be cultured both in basal media supplemented with fetal bovine 
      serum (FBS) and in serum-free media. In this work, the supplementation of Grace's 
      medium with a set of nutrients to reduce FBS requirements in Spodoptera 
      frugiperda (Sf9) cell culture was evaluated, aiming the production of Anticarsia 
      gemmatalis nucleopolyhedrovirus (AgMNPV) at a cost lower than those for the 
      production using Sf900 II medium. In Grace's medium supplemented with glucose, 
      Pluronic F68 (PF68) and yeast extract (YE), the effects of FBS and milk whey 
      ultrafiltrate (MWU) on cell concentration and viability during midexponential and 
      stationary growth phase were evaluated. In spite of the fact that FBS presented 
      higher statistical effects than MWU on all dependent variables in the first cell 
      passage studies, after cell adaptation, AgMNPV polyhedra production was 
      comparable to that in Sf900 II. Batch cultivation in Grace's medium with 2.7 g 
      l(-1) glucose, 8 g l(-1) YE and 0.1% (w/v) PF68 supplemented with 1% (w/v) MWU 
      and 3% (v/v) FBS increased viable cell concentration to about 5-fold (4.7x10(6) 
      cells ml(-1)) when compared to Grace's containing 10% (v/v) FBS (9.5x10(5) cells 
      ml(-1)). AgMNPV polyhedra (PIBs) production was around 3-fold higher in the MWU 
      supplemented medium (1.6x10(7) PIBs ml(-1)) than in Grace's medium with 10% FBS 
      (0.6x10(7) PIBs ml(-1)). This study therefore shows a promising achievement to 
      significantly reduce FBS concentration in Sf9 insect cell media, keeping high 
      productivity in terms of cell concentration and final virus production at a cost 
      almost 50% lower than that observed for Sf900 II medium.
FAU - Batista, Fabiana R X
AU  - Batista FR
AD  - Departamento de Processos Biotecnologicos, Faculdade de Engenharia Quimca, 
      Universidade Estadual de Campinas, P.O. Box 6066, 13083-852, Campinas, SP, 
      Brazil.
FAU - Pereira, Carlos A
AU  - Pereira CA
FAU - Mendonca, Ronaldo Z
AU  - Mendonca RZ
FAU - Moraes, Angela M
AU  - Moraes AM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC3449745
EDAT- 2008/11/13 09:00
MHDA- 2008/11/13 09:01
PMCR- 2006/09/01
CRDT- 2008/11/13 09:00
PHST- 2005/04/27 00:00 [received]
PHST- 2005/10/19 00:00 [accepted]
PHST- 2008/11/13 09:00 [pubmed]
PHST- 2008/11/13 09:01 [medline]
PHST- 2008/11/13 09:00 [entrez]
PHST- 2006/09/01 00:00 [pmc-release]
AID - 4206 [pii]
AID - 10.1007/s10616-005-4206-0 [doi]
PST - ppublish
SO  - Cytotechnology. 2005 Sep;49(1):1-9. doi: 10.1007/s10616-005-4206-0.