PMID- 19003130
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20211020
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 31
IP  - 1-2
DP  - 1999 Sep
TI  - Productivity enhancement of recombinant protein in CHO cells via specific 
      promoter activation by oncogenes.
PG  - 103-9
LID - 10.1023/A:1008048928053 [doi]
AB  - To construct a recombinant protein highly producing cell lines, we have 
      previously developed the Oncogene Activated Production (OAP) system by using 
      BHK-21 cells. Here we verified the availability of the OAP system in CHO cells. 
      We firstly generated 'primed' ras amplified CHO cells, ras clone I, by 
      introducing human c-Ha-ras oncogene into CHO cells. This ras clone I enables 
      quick and easy establishment of recombinant protein hyper producing cell lines by 
      introduction reporter gene of interest. Then we generated I13 by introducing 
      human interleukin 6 (hIL-6) gene as a reporter gene, which showed enhanced 
      productivity rate as compared to A7 established by conventional method. 
      Furthermore, we found that hIL-6 production level of I13 was slightly improved by 
      raising the CO(2) concentration from 5 to 8% possibly because of the enhanced 
      growth rate. We further introduced the E1A oncogene, which has been shown to have 
      a synergistic effect on the recombinant protein production of the ras-amplified 
      BHK-21 cells, then evaluated the productivity. When culture in 5% CO(2) 
      condition, only the slight effect can be seen. However when cultured in 8% CO(2) 
      condition, not only cell number, but also productivity increased significantly, 
      resulted in great augmentation of hIL-6 production, maximum production being 88.6 
      mug/ml/3 days. This study demonstrates that recombinant protein production level 
      reached commercially desirable level by utilizing our OAP system in CHO cells and 
      optimizing the culture condition.
FAU - Katakura, Y
AU  - Katakura Y
FAU - Seto, P
AU  - Seto P
FAU - Miura, T
AU  - Miura T
FAU - Ohashi, H
AU  - Ohashi H
FAU - Teruya, K
AU  - Teruya K
FAU - Shirahata, S
AU  - Shirahata S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC3449785
EDAT- 2008/11/13 09:00
MHDA- 2008/11/13 09:01
PMCR- 2000/09/01
CRDT- 2008/11/13 09:00
PHST- 2008/11/13 09:00 [entrez]
PHST- 2008/11/13 09:00 [pubmed]
PHST- 2008/11/13 09:01 [medline]
PHST- 2000/09/01 00:00 [pmc-release]
AID - 211143 [pii]
AID - 10.1023/A:1008048928053 [doi]
PST - ppublish
SO  - Cytotechnology. 1999 Sep;31(1-2):103-9. doi: 10.1023/A:1008048928053.