PMID- 19003308
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 39
IP  - 3
DP  - 2002 Sep
TI  - Effect of an exo-polysaccharide from the culture broth of Hericium erinaceus on 
      enhancement of growth and differentiation of rat adrenal nerve cells.
PG  - 155-62
LID - 10.1023/A:1023963509393 [doi]
AB  - It was found that an exo-biopolymer (M.W. 1,000,000, molar ratio of 
      1.5:1.7:1.2:0.6:0.9, glucose:galactose:xylose:mannose:fructose, purity 99%) 
      purified from the liquid culture broth of Hericium erinaceus mycelium enhanced 
      the growth of rat adrenal nerve cells. The polymer also improved the extension of 
      the neurites of PC12 cell. Its efficacy was found to be higher than those from 
      known nerve growth factors such as Nerve Growth Factor (NGF) and Brain-Derived 
      Nerve Factor (BDNF). The effect of two standards has not been observed above 0.1 
      (mg l(-1)) of supplementation; however, the polymer did show the effect of cell 
      growth and neurite extension at up to 1.0 (mg l(-1)) of addition. While the 
      polymer improved both cell growth and neurite extension, NGF and BDNF did only 
      outgrowth of the neurites. Maximum cell density and length of the neurites were 
      observed as 1.5x10(5) (viable cells ml(-1)) and 230 mum, respectively in adding 
      0.8 (mg l(-1)) of the biopolymer for 8 days cultivation. The control growth was 
      observed only as 1.2x10(5) (viable cell ml(-1)) of maximum cell density and 140 
      mum of maximum length, respectively. It was also confirmed that the polymer 
      reacted with the nerve cells within 30 min after adding the sample, compared to 
      80 min in adding two other growth factors. Number of neurite-bearing cells 
      remained relatively steady in adding the polymer even when the cell growth 
      started to be decreased. It was interesting that the polymer effectively delayed 
      apoptosis of PC12 cells by dramatically reducing the ratio of apoptotic cells to 
      20% from 50% of the control.
FAU - Park, Young Shik
AU  - Park YS
AD  - School of Biotechnology and Bioengineering, Kangwon National University, 
      Chunchon, 200-701, S. Korea.
FAU - Lee, Hyun Soo
AU  - Lee HS
FAU - Won, Moo Ho
AU  - Won MH
FAU - Lee, Jin Ha
AU  - Lee JH
FAU - Lee, Shin Young
AU  - Lee SY
FAU - Lee, Hyeon Yong
AU  - Lee HY
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC3449638
EDAT- 2008/11/13 09:00
MHDA- 2008/11/13 09:01
PMCR- 2003/09/01
CRDT- 2008/11/13 09:00
PHST- 2008/11/13 09:00 [pubmed]
PHST- 2008/11/13 09:01 [medline]
PHST- 2008/11/13 09:00 [entrez]
PHST- 2003/09/01 00:00 [pmc-release]
AID - 5102359 [pii]
AID - 10.1023/A:1023963509393 [doi]
PST - ppublish
SO  - Cytotechnology. 2002 Sep;39(3):155-62. doi: 10.1023/A:1023963509393.