PMID- 19003396
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 34
IP  - 3
DP  - 2000 Nov
TI  - Growing colorectal tumors: minimizing microbial and stromal competition and 
      assessing in vitro selection pressures.
PG  - 205-11
LID - 10.1023/A:1008131428992 [doi]
AB  - Development of primary colorectal cancer cell lines ishampered by contamination 
      from regional microbes, overgrowthof stromal cells, and purported genetic drift 
      from selectionpressures in vitro. We initiated 32 primaryadenocarcinomas, 3 
      recurrences and 6 distant metastases incell culture. Twelve cell lines from 
      eleven tumors weregenerated (26.8%) overall. Nine of 32 primary tumorsyielded 10 
      cell lines, 5 were lost to contamination, 13 wereoverwhelmed by stromal cells, 
      and 5 demonstrated no growth.Addition of isobutyl methyl xanthine (IBMX) to 
      culturelimited fibroblastoid growth. There was no associationbetween tumor 
      location (p = 0.535, mid-P), degree ofdifferentiation (p = 0.850, mid-P) or 
      clinicopathologic stage(p = 0.400, mid-P), and the ability of cells to 
      becomeestablished in culture. The majority of cell lines hadsimilar nuclear DNA 
      content and expression of cell-surfaceantigens compared with their parent tumors. 
      Microbialcontamination and stromal cell overgrowth present thegreatest obstacle 
      to capturing a representative bank ofcolon tumors in vitro.
FAU - Farrell, T M
AU  - Farrell TM
AD  - Departments of Surgery and Pathology, Dartmouth-Hitchcock Medical Center, 
      Lebanon, NH, U.S.A..
FAU - Pettengill, O S
AU  - Pettengill OS
FAU - Longnecker, D S
AU  - Longnecker DS
FAU - Cate, C C
AU  - Cate CC
FAU - Cohn, K H
AU  - Cohn KH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC3449633
EDAT- 2008/11/13 09:00
MHDA- 2008/11/13 09:01
PMCR- 2001/11/01
CRDT- 2008/11/13 09:00
PHST- 2008/11/13 09:00 [pubmed]
PHST- 2008/11/13 09:01 [medline]
PHST- 2008/11/13 09:00 [entrez]
PHST- 2001/11/01 00:00 [pmc-release]
AID - 276709 [pii]
AID - 10.1023/A:1008131428992 [doi]
PST - ppublish
SO  - Cytotechnology. 2000 Nov;34(3):205-11. doi: 10.1023/A:1008131428992.