PMID- 19004848
OWN - NLM
STAT- MEDLINE
DCOM- 20090807
LR  - 20151119
IS  - 1460-2385 (Electronic)
IS  - 0931-0509 (Linking)
VI  - 24
IP  - 4
DP  - 2009 Apr
TI  - Serum cystatin C in mouse models: a reliable and precise marker for renal 
      function and superior to serum creatinine.
PG  - 1157-61
LID - 10.1093/ndt/gfn626 [doi]
AB  - BACKGROUND: Serum creatinine (SCR) and blood urea nitrogen (BUN) determine the 
      glomerular filtration rate (GFR) improperly in acute renal failure. Serum 
      cystatin C (CYS) has the potential to be a more precise marker for GFR. The aim 
      of this study was to compare the sensitivity of SCR, BUN and CYS with respect to 
      the detection of acute renal failure in mice. METHODS: In an ischaemia 
      reperfusion (I/R) injury model, mice suffered 60-min left kidney ischaemia and 
      right nephrectomy. In a nephrectomy model, mice were nephrectomized to a 
      different extent: from unilateral (3/6Nx) to bilateral nephrectomy (BiNx). Blood 
      samples were collected 2, 12 or 24 h post-op. RESULTS: SCR, BUN and CYS increased 
      significantly in the I/R-model in comparison to sham mice and 3/6Nx mice at 12 
      and 24 h post-op (SCR P = 0.009; BUN P < 0.001 and CYS P < 0.004). There were no 
      significant differences in all three markers between 3/6Nx and sham-operated 
      mice. In graded nephrectomy, BUN and CYS showed already significantly the loss of 
      kidney in 4/6Nx mice 12 h post-op [BUN (mg/dl): sham 26.4 +/- 3.5, 4/6Nx 52.3 +/- 
      13.4, P < 0.01; CYS (mg/l): sham 0.08 +/- 0.03, 4/6Nx 0.15 +/- 0.04, P < 0.01], 
      whereas SCR was only significantly increased in 5/6Nx and BiNx mice 24 h post-op 
      [SCR (mg/dl): sham 0.39 +/- 0.05, 4/6Nx 0.52 +/- 0.07, P = 0.13, 5/6Nx 1.00 +/- 
      0.29, P < 0.01]. In the longitudinal experiment, CYS showed the renal damage 
      significantly earlier and to a larger extent (2 h: SCR 57 +/- 15%, BUN 40 +/- 
      16%, CYS 295 +/- 143%, P <0.001). CONCLUSIONS: CYS can be used as a reliable and 
      precise marker for renal function in mouse models. CYS is more sensitive than 
      SCR, and it shows renal damage earlier than SCR and BUN.
FAU - Song, Su
AU  - Song S
AD  - Department of Nephrology, University Hospital Essen, University of 
      Duisburg-Essen, Hufelandstrasse 55, 45122 Essen, Germany.
FAU - Meyer, Marko
AU  - Meyer M
FAU - Turk, Tobias R
AU  - Turk TR
FAU - Wilde, Benjamin
AU  - Wilde B
FAU - Feldkamp, Thorsten
AU  - Feldkamp T
FAU - Assert, Roland
AU  - Assert R
FAU - Wu, Kun
AU  - Wu K
FAU - Kribben, Andreas
AU  - Kribben A
FAU - Witzke, Oliver
AU  - Witzke O
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20081111
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Biomarkers)
RN  - 0 (Cystatin C)
RN  - AYI8EX34EU (Creatinine)
SB  - IM
MH  - Acute Kidney Injury/*blood/*diagnosis/etiology
MH  - Animals
MH  - Biomarkers/blood
MH  - Blood Urea Nitrogen
MH  - Creatinine/blood
MH  - Cystatin C/*blood
MH  - Disease Models, Animal
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Reperfusion Injury/*blood/complications
EDAT- 2008/11/14 09:00
MHDA- 2009/08/08 09:00
CRDT- 2008/11/14 09:00
PHST- 2008/11/14 09:00 [pubmed]
PHST- 2009/08/08 09:00 [medline]
PHST- 2008/11/14 09:00 [entrez]
AID - gfn626 [pii]
AID - 10.1093/ndt/gfn626 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2009 Apr;24(4):1157-61. doi: 10.1093/ndt/gfn626. Epub 
      2008 Nov 11.