PMID- 19004935
OWN - NLM
STAT- MEDLINE
DCOM- 20090126
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 83
IP  - 2
DP  - 2009 Jan
TI  - Structural and functional studies of the promoter element for dengue virus RNA 
      replication.
PG  - 993-1008
LID - 10.1128/JVI.01647-08 [doi]
AB  - The 5' untranslated region (5'UTR) of the dengue virus (DENV) genome contains two 
      defined elements essential for viral replication. At the 5' end, a large 
      stem-loop (SLA) structure functions as the promoter for viral polymerase 
      activity. Next to the SLA, there is a short stem-loop that contains a cyclization 
      sequence known as the 5' upstream AUG region (5'UAR). Here, we analyzed the 
      secondary structure of the SLA in solution and the structural requirements of 
      this element for viral replication. Using infectious DENV clones, viral 
      replicons, and in vitro polymerase assays, we defined two helical regions, a side 
      stem-loop, a top loop, and a U bulge within SLA as crucial elements for viral 
      replication. The determinants for SLA-polymerase recognition were found to be 
      common in different DENV serotypes. In addition, structural elements within the 
      SLA required for DENV RNA replication were also conserved among different 
      mosquito- and tick-borne flavivirus genomes, suggesting possible common 
      strategies for polymerase-promoter recognition in flaviviruses. Furthermore, a 
      conserved oligo(U) track present downstream of the SLA was found to modulate RNA 
      synthesis in transfected cells. In vitro polymerase assays indicated that a 
      sequence of at least 10 residues following the SLA, upstream of the 5'UAR, was 
      necessary for efficient RNA synthesis using the viral 3'UTR as template.
FAU - Lodeiro, Maria F
AU  - Lodeiro MF
AD  - Fundacion Instituto Leloir, Buenos Aires, Argentina.
FAU - Filomatori, Claudia V
AU  - Filomatori CV
FAU - Gamarnik, Andrea V
AU  - Gamarnik AV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081112
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cricetinae
MH  - Dengue Virus/genetics/*physiology
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - *Promoter Regions, Genetic
MH  - RNA, Viral/*biosynthesis/*chemistry/genetics
MH  - *Virus Replication
PMC - PMC2612346
EDAT- 2008/11/14 09:00
MHDA- 2009/01/27 09:00
PMCR- 2009/07/01
CRDT- 2008/11/14 09:00
PHST- 2008/11/14 09:00 [pubmed]
PHST- 2009/01/27 09:00 [medline]
PHST- 2008/11/14 09:00 [entrez]
PHST- 2009/07/01 00:00 [pmc-release]
AID - JVI.01647-08 [pii]
AID - 1647-08 [pii]
AID - 10.1128/JVI.01647-08 [doi]
PST - ppublish
SO  - J Virol. 2009 Jan;83(2):993-1008. doi: 10.1128/JVI.01647-08. Epub 2008 Nov 12.