PMID- 19004989
OWN - NLM
STAT- MEDLINE
DCOM- 20090311
LR  - 20171116
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 85
IP  - 2
DP  - 2009 Feb
TI  - Salmonella induces death of CD8alpha(+) dendritic cells but not 
      CD11c(int)CD11b(+) inflammatory cells in vivo via MyD88 and TNFR1.
PG  - 225-34
LID - 10.1189/jlb.0708413 [doi]
AB  - Dendritic cells (DCs), whose lifespan influences their ability to stimulate the 
      immune system, are potent APCs that are critical for initiating immunity. Here, 
      we show that oral infection with Salmonella enterica serovar Typhimurium induces 
      death of DCs in the gut-draining lymph nodes. Although CD8alpha(+) DCs were 
      sensitive to Salmonella-induced death, CD8alpha(-) DCs and in particular 
      recruited CD11c(int)CD11b(+) inflammatory cells, were resistant. Infecting mice 
      deficient for MyD88 revealed that Salmonella-induced death of CD8alpha(+) DCs was 
      dependent on this adaptor for TLR signaling. In addition, CD8alpha(+) DCs in 
      infected, TNFR1-deficient mice were resistant to Salmonella-induced death. These 
      data, combined with the strict MyD88-dependent production of TNF in 
      Salmonella-infected mice, suggest that MyD88-dependent TNF mediates DC death. As 
      recruited CD11c(int)CD11b(+) cells were resistant to Salmonella-induced death, 
      they could compensate for the infection-induced loss of DCs if they function as 
      APCs. However, in contrast to DCs, CD11c(int)CD11b(+) cells could not present the 
      model antigen OVA expressed in Salmonella to OVA-specific CD4 T cells. These 
      results show that Salmonella induces DC death after oral infection via MyD88 and 
      TNFR1, which could have a negative impact on the initiation of antibacterial 
      immunity.
FAU - Sundquist, Malin
AU  - Sundquist M
AD  - Department of Microbiology and Immunology, Goteborg University, Goteborg, Sweden.
FAU - Wick, Mary Jo
AU  - Wick MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081112
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (CD11b Antigen)
RN  - 0 (CD11c Antigen)
RN  - 0 (CD8 Antigens)
RN  - 0 (CD8 antigen, alpha chain)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/immunology
MH  - CD11b Antigen/*immunology
MH  - CD11c Antigen/*immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8 Antigens/*immunology
MH  - Cell Count
MH  - Cell Death
MH  - Dendritic Cells/*cytology/immunology/microbiology
MH  - Green Fluorescent Proteins/metabolism
MH  - Inflammation/immunology/microbiology
MH  - Lymph Nodes/cytology/immunology/microbiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Myeloid Differentiation Factor 88/deficiency/*immunology
MH  - Receptors, Tumor Necrosis Factor, Type I/*immunology
MH  - Salmonella Infections/immunology
MH  - Salmonella enterica/*immunology
EDAT- 2008/11/14 09:00
MHDA- 2009/03/12 09:00
CRDT- 2008/11/14 09:00
PHST- 2008/11/14 09:00 [pubmed]
PHST- 2009/03/12 09:00 [medline]
PHST- 2008/11/14 09:00 [entrez]
AID - jlb.0708413 [pii]
AID - 10.1189/jlb.0708413 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2009 Feb;85(2):225-34. doi: 10.1189/jlb.0708413. Epub 2008 Nov 12.