PMID- 19005487
OWN - NLM
STAT- MEDLINE
DCOM- 20090501
LR  - 20151119
IS  - 1523-1747 (Electronic)
IS  - 0022-202X (Linking)
VI  - 129
IP  - 5
DP  - 2009 May
TI  - Skin exposure to weak and moderate contact allergens induces IFNgamma production 
      by lymph node cells of CD4+ T-cell-depleted mice.
PG  - 1185-91
LID - 10.1038/jid.2008.352 [doi]
AB  - Allergic contact dermatitis (ACD) is mediated by hapten-specific CD8+ T cells and 
      downregulated by CD4+ T cells. We have recently shown in a model of ACD to weak 
      haptens that priming of IFNgamma-producing CD8+ T cells and the development of 
      skin inflammation could be obtained in mice deficient in CD4+ T cells. Here we 
      show that IFNgamma production by lymph node (LN) cells draining the site of skin 
      sensitization of CD4+ T-cell-deficient mice is a marker of the sensitizing 
      properties of weak haptens. LN cells from mice sensitized as in the classical 
      local lymph node assay (LLNA) were recovered at day 5, then cultured for 20 hours 
      in the presence of submitogenic doses of phytohemagglutinin, and finally tested 
      for the production of IFNgamma. Results show that: (i) production of INFgamma by 
      LN cells was induced by weak and moderate allergens in a dose-dependent fashion; 
      (ii) the magnitude of IFNgamma production paralleled the sensitizing properties 
      of allergens allowing to classify them as moderate or weak haptens; (iii) 
      chemicals without sensitizing properties were unable to stimulate IFNgamma 
      production by LN cells. Therefore, the IFNgamma LLNA appears as a sensitive, 
      specific, and robust assay to detect weak contact allergens.
FAU - Vocanson, Marc
AU  - Vocanson M
AD  - Faculte de Medecine, Universite Lyon1, Lyon-Sud, France. marc.vocanson@inserm.fr
FAU - Hennino, Anca
AU  - Hennino A
FAU - Rozieres, Aurore
AU  - Rozieres A
FAU - Cluzel-Tailhardat, Magalie
AU  - Cluzel-Tailhardat M
FAU - Poyet, Gaelle
AU  - Poyet G
FAU - Valeyrie, Magalie
AU  - Valeyrie M
FAU - Benetiere, Josette
AU  - Benetiere J
FAU - Tedone, Rosine
AU  - Tedone R
FAU - Kaiserlian, Dominique
AU  - Kaiserlian D
FAU - Nicolas, Jean-Francois
AU  - Nicolas JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081113
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Allergens)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Biomarkers)
RN  - 0 (Haptens)
RN  - 0 (Phytohemagglutinins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Allergens/immunology/*pharmacology
MH  - Animals
MH  - Antibodies, Monoclonal/immunology/pharmacology
MH  - Biomarkers/metabolism
MH  - CD4-Positive T-Lymphocytes/drug effects/*pathology
MH  - Dermatitis, Allergic Contact/immunology/metabolism/pathology
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Haptens/immunology/pharmacology
MH  - Interferon-gamma/*metabolism
MH  - *Local Lymph Node Assay
MH  - Lymph Nodes/immunology/*metabolism/pathology
MH  - Lymphocyte Depletion/methods
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Phytohemagglutinins/pharmacology
MH  - Sensitivity and Specificity
MH  - Skin/*drug effects/*immunology
EDAT- 2008/11/14 09:00
MHDA- 2009/05/02 09:00
CRDT- 2008/11/14 09:00
PHST- 2008/11/14 09:00 [pubmed]
PHST- 2009/05/02 09:00 [medline]
PHST- 2008/11/14 09:00 [entrez]
AID - S0022-202X(15)34343-8 [pii]
AID - 10.1038/jid.2008.352 [doi]
PST - ppublish
SO  - J Invest Dermatol. 2009 May;129(5):1185-91. doi: 10.1038/jid.2008.352. Epub 2008 
      Nov 13.