PMID- 19008128
OWN - NLM
STAT- MEDLINE
DCOM- 20081229
LR  - 20081205
IS  - 1532-2165 (Electronic)
IS  - 1078-5884 (Linking)
VI  - 37
IP  - 1
DP  - 2009 Jan
TI  - Characterisation of Interleukin-8 and monocyte chemoattractant protein-1 
      expression within the abdominal aortic aneurysm and their association with mural 
      inflammation.
PG  - 46-55
LID - 10.1016/j.ejvs.2008.09.013 [doi]
AB  - OBJECTIVES: Abdominal aortic aneurysms (AAAs) are characterised by chronic 
      transmural inflammation. This study investigated the expression of interleukin-8 
      (IL-8) and monocyte chemoattractant protein-1 (MCP-1) within the AAA, and their 
      relationship with mural inflammation. METHODS: Biopsies were obtained from 25 
      AAAs, 15 abdominal aortas, and 10 atherosclerotic thoracic aortas. IL-8 and MCP-1 
      expression was measured in homogenised specimens by ELISA. Infiltrate composition 
      and localised expression of IL-8 and MCP-1 were determined through 
      immunohistochemistry. RESULTS: ELISA analysis demonstrated that IL-8 and MCP-1 
      were raised in the AAA compared to the controls [(IL-8, AAA vs. abdominal aorta: 
      >28-fold, P<.001; AAA vs. thoracic aorta: >28-fold, P<.001) (MCP-1, AAA vs. 
      abdominal aorta: 9-fold, P<.001; AAA vs. thoracic aorta: 19-fold, P<.001)]. 
      Immunohistochemistry revealed that IL-8 was localised to the inflammatory 
      infiltrate, which consisted predominantly of CD3(+) T- and CD20(+) B-lymphocytes. 
      MCP-1 was predominantly expressed by CD68(+) macrophages. Increasing IL-8 
      expression was associated with an increase in mural inflammation, and an increase 
      in CD3(+) T-lymphocytes of CD4(+) phenotype within the infiltrate population. 
      CONCLUSION: Pathways involving IL-8 and MCP-1 may be involved in AAA 
      pathogenesis. IL-8 may be directly involved in the chemotaxis of T(H)-lymphocytes 
      into the AAA wall.
FAU - Middleton, R K
AU  - Middleton RK
AD  - Department of Cardiovascular Sciences, Vascular Surgery Group, University of 
      Leicester, Leicester, UK. rk_middleton@yahoo.co.uk
FAU - Bown, M J
AU  - Bown MJ
FAU - Lloyd, G M
AU  - Lloyd GM
FAU - Jones, J L
AU  - Jones JL
FAU - London, N J
AU  - London NJ
FAU - Sayers, R D
AU  - Sayers RD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081112
PL  - England
TA  - Eur J Vasc Endovasc Surg
JT  - European journal of vascular and endovascular surgery : the official journal of 
      the European Society for Vascular Surgery
JID - 9512728
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aortic Aneurysm, Abdominal/*immunology/pathology
MH  - Chemokine CCL2/*biosynthesis/immunology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-8/*biosynthesis/immunology
MH  - Lymphocytes/*immunology
MH  - Male
MH  - Middle Aged
MH  - Young Adult
EDAT- 2008/11/15 09:00
MHDA- 2008/12/30 09:00
CRDT- 2008/11/15 09:00
PHST- 2008/06/01 00:00 [received]
PHST- 2008/09/19 00:00 [accepted]
PHST- 2008/11/15 09:00 [pubmed]
PHST- 2008/12/30 09:00 [medline]
PHST- 2008/11/15 09:00 [entrez]
AID - S1078-5884(08)00518-2 [pii]
AID - 10.1016/j.ejvs.2008.09.013 [doi]
PST - ppublish
SO  - Eur J Vasc Endovasc Surg. 2009 Jan;37(1):46-55. doi: 10.1016/j.ejvs.2008.09.013. 
      Epub 2008 Nov 12.