PMID- 19008311
OWN - NLM
STAT- MEDLINE
DCOM- 20090324
LR  - 20090225
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Linking)
VI  - 150
IP  - 3
DP  - 2009 Mar
TI  - Differential neuroendocrine expression of multiple brain-derived neurotrophic 
      factor transcripts.
PG  - 1361-8
LID - 10.1210/en.2008-0993 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is a neurotrophin with important 
      growth-promoting properties. We report here the first characterization of a BDNF 
      gene in an amphibian, Xenopus laevis, and demonstrate that environmental factors 
      can activate this gene in a promoter-specific fashion. The Xenopus BDNF gene 
      contains six promoter-specific 5'-exons and one 3'-protein-encoding exon. We 
      examined the expression of promoter-specific transcripts in Xenopus 
      neuroendocrine melanotrope cells. These cells make a good model to study how 
      environmental factors control gene expression. In animals placed on a black 
      background melanotrope cells more actively produce and release alphaMSH than in 
      animals on a white background. BDNF is cosequestered and coreleased with alphaMSH 
      and stimulates biosynthesis of proopiomelanocortin (POMC), the precursor protein 
      for alphaMSH. Our analysis of the expression of the BDNF transcripts revealed 
      that there is differential use of some BDNF promoters in melanotrope cells, 
      depending on the adaptation state of the frog. During black-background 
      adaptation, stimulation of expression of BDNF transcript IV preceded that of the 
      POMC transcript, suggesting the BDNF gene is an effector gene for POMC 
      expression. The possible mechanisms regulating expression of the various 
      transcripts are discussed on the basis of the potential calcium- and 
      cAMP-responsive elements in the promoter region of exon IV. Finally, we show that 
      the upstream open reading frames of BDNF transcripts I and IV markedly decrease 
      BDNF translation efficiency, giving the first indication for a functional role of 
      untranslated BDNF exons.
FAU - Kidane, Adhanet H
AU  - Kidane AH
AD  - Department of Cellular Animal Physiology, Radboud University Nijmegen, Nijmegen, 
      The Netherlands.
FAU - Heinrich, Gerhard
AU  - Heinrich G
FAU - Dirks, Ron P H
AU  - Dirks RP
FAU - de Ruyck, Brechje A
AU  - de Ruyck BA
FAU - Lubsen, Nicolette H
AU  - Lubsen NH
FAU - Roubos, Eric W
AU  - Roubos EW
FAU - Jenks, Bruce G
AU  - Jenks BG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081113
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (RNA, Messenger)
RN  - 66796-54-1 (Pro-Opiomelanocortin)
SB  - IM
MH  - Adaptation, Physiological/genetics
MH  - Animals
MH  - Base Sequence
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Cloning, Molecular
MH  - Color
MH  - *Gene Expression Regulation
MH  - Molecular Sequence Data
MH  - Neuroendocrine Cells/*metabolism
MH  - Organ Specificity/genetics
MH  - Pro-Opiomelanocortin/genetics
MH  - Protein Biosynthesis/physiology
MH  - Proto-Oncogene Proteins c-fos/genetics
MH  - RNA, Messenger/metabolism
MH  - Regulatory Elements, Transcriptional/genetics
MH  - Time Factors
MH  - Xenopus laevis/genetics
EDAT- 2008/11/15 09:00
MHDA- 2009/03/25 09:00
CRDT- 2008/11/15 09:00
PHST- 2008/11/15 09:00 [pubmed]
PHST- 2009/03/25 09:00 [medline]
PHST- 2008/11/15 09:00 [entrez]
AID - en.2008-0993 [pii]
AID - 10.1210/en.2008-0993 [doi]
PST - ppublish
SO  - Endocrinology. 2009 Mar;150(3):1361-8. doi: 10.1210/en.2008-0993. Epub 2008 Nov 
      13.