PMID- 19009624
OWN - NLM
STAT- MEDLINE
DCOM- 20090203
LR  - 20211020
IS  - 1096-9861 (Electronic)
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 512
IP  - 3
DP  - 2009 Jan 20
TI  - Identification and characterization of pannexin expression in the mammalian 
      cochlea.
PG  - 336-46
LID - 10.1002/cne.21898 [doi]
AB  - The gap junction in vertebrates is encoded by the connexin gene family. Recently, 
      a new gene family termed pannexin (Panx) has been identified in vertebrates and 
      found to encode gap junctional proteins as well. To date, three pannexin isoforms 
      (Panx1, 2, and 3) have been cloned from mouse and human genomes. In this study, 
      expression of pannexins in the mouse and rat cochlea was investigated. Polymerase 
      chain reaction and Western blot analysis showed that all three pannexin isoforms 
      were expressed in the cochlea. Immunofluorescent staining showed that Panx1 
      expression was extensive. In the organ of Corti, Panx1 labeling was found in 
      supporting cells, including pillar cells, Hensen cells, Claudius cells, and 
      Boettcher cells. Both surface plaque-like punctate labeling and 
      diffuse-cytoplasmic labeling were visible. However, the labeling was weak and 
      rare in Deiters cells. No labeling was found in the hair cells. Intense labeling 
      for Panx1 was also observed in the interdental cells in the spiral limbus, the 
      inner and outer sulcus cells, and the type II fibrocytes in the spiral prominence 
      and central region in the cochlear lateral wall. In addition, Panx1 labeling was 
      detectable in Reissner's membrane and strial blood vessel cells. Panx2 labeling 
      was restricted to the basal cells in the stria vascularis and was also detectable 
      in the spiral ganglion neurons. However, no overlapping labeling for Panx1 and 
      Panx2 was observed. Finally, Panx3 labeling was exclusively observed in the 
      cochlear bone. Thus, Panx1, 2, and 3 are abundantly expressed in the mammalian 
      cochlea and demonstrate distinct cellular distributions. Like connexins, they may 
      play an important role in hearing.
FAU - Wang, Xiao-Hui
AU  - Wang XH
AD  - Department of Surgery-Otolaryngology, University of Kentucky Medical Center, 
      Lexington, Kentucky 40536, USA.
FAU - Streeter, Michele
AU  - Streeter M
FAU - Liu, Ying-Peng
AU  - Liu YP
FAU - Zhao, Hong-Bo
AU  - Zhao HB
LA  - eng
GR  - R01 DC005989/DC/NIDCD NIH HHS/United States
GR  - R01 DC005989-05/DC/NIDCD NIH HHS/United States
GR  - R01 DC05989/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Connexins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Panx1 protein, mouse)
RN  - 0 (Panx2 protein, mouse)
RN  - 0 (Protein Isoforms)
RN  - 0 (pannexin 1, rat)
RN  - 0 (pannexin 2, rat)
RN  - 0 (pannexin 3 protein, mouse)
RN  - 0 (pannexin 3, rat)
SB  - IM
EIN - J Comp Neurol. 2010 Apr 1;518(7):1156. Dosage error in article text
MH  - Animals
MH  - *Cochlea/anatomy & histology/metabolism
MH  - Connexins/genetics/*metabolism
MH  - Gap Junctions/metabolism/ultrastructure
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Protein Isoforms/genetics/*metabolism
MH  - Rats
PMC - PMC2630187
MID - NIHMS72124
EDAT- 2008/11/15 09:00
MHDA- 2009/02/04 09:00
PMCR- 2010/01/20
CRDT- 2008/11/15 09:00
PHST- 2008/11/15 09:00 [pubmed]
PHST- 2009/02/04 09:00 [medline]
PHST- 2008/11/15 09:00 [entrez]
PHST- 2010/01/20 00:00 [pmc-release]
AID - 10.1002/cne.21898 [doi]
PST - ppublish
SO  - J Comp Neurol. 2009 Jan 20;512(3):336-46. doi: 10.1002/cne.21898.