PMID- 19010703
OWN - NLM
STAT- MEDLINE
DCOM- 20100303
LR  - 20100201
IS  - 1532-2971 (Electronic)
IS  - 1090-0233 (Linking)
VI  - 183
IP  - 2
DP  - 2010 Feb
TI  - The stability of the reactive oxygen metabolites (d-ROMs) and biological 
      antioxidant potential (BAP) tests on stored horse blood.
PG  - 217-8
LID - 10.1016/j.tvjl.2008.09.018 [doi]
AB  - Increasing interest in the role of oxidative stress (OS) in equine medicine has 
      highlighted the need to develop reliable methods to quantify it. In this study we 
      describe the effect of refrigeration (at 4 degrees C) on the stability of the 
      reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) 
      tests carried out on 15 healthy horses. Blood samples, collected from the jugular 
      vein, were immediately placed on ice and analysed using both the d-ROMs and BAP 
      tests. Samples were also refrigerated at 4 degrees C and tested after 3, 7 and 24 
      h. The average results were similar for up to 24 h and minimal variations were 
      found for each horse. The findings suggest that refrigeration is suitable for 
      preserving equine blood samples for these assays and this approach will provide 
      veterinarians with a technically simple, reliable test to measure OS under field 
      conditions.
CI  - Copyright (c) 2008 Elsevier Ltd. All rights reserved.
FAU - Celi, P
AU  - Celi P
AD  - Faculty of Veterinary Science, The University of Sydney, PMB 3, Camden NSW, 2570, 
      Australia. pietroc@camden.usyd.edu.au
FAU - Sullivan, M
AU  - Sullivan M
FAU - Evans, D
AU  - Evans D
LA  - eng
PT  - Journal Article
DEP - 20081117
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
RN  - 0 (Antioxidants)
RN  - 0 (Free Radicals)
SB  - IM
MH  - Animals
MH  - Antioxidants/*metabolism
MH  - Free Radicals/blood
MH  - Horses/*blood
MH  - Oxidation-Reduction
MH  - *Oxidative Stress/physiology
MH  - Refrigeration/veterinary
MH  - Specimen Handling/veterinary
EDAT- 2008/11/18 09:00
MHDA- 2010/03/04 06:00
CRDT- 2008/11/18 09:00
PHST- 2008/06/22 00:00 [received]
PHST- 2008/09/29 00:00 [revised]
PHST- 2008/09/30 00:00 [accepted]
PHST- 2008/11/18 09:00 [pubmed]
PHST- 2010/03/04 06:00 [medline]
PHST- 2008/11/18 09:00 [entrez]
AID - S1090-0233(08)00342-0 [pii]
AID - 10.1016/j.tvjl.2008.09.018 [doi]
PST - ppublish
SO  - Vet J. 2010 Feb;183(2):217-8. doi: 10.1016/j.tvjl.2008.09.018. Epub 2008 Nov 17.