PMID- 19012739
OWN - NLM
STAT- MEDLINE
DCOM- 20090224
LR  - 20211020
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 108
IP  - 3
DP  - 2009 Feb
TI  - Fibronectin stimulates TRPV1 translocation in primary sensory neurons.
PG  - 591-600
LID - 10.1111/j.1471-4159.2008.05779.x [doi]
AB  - Extracellular matrix (ECM) molecules are highly variable in their composition and 
      receptor recognition. Their ubiquitous expression profile has been linked to 
      roles in cell growth, differentiation, and survival. Recent work has identified 
      certain ECM molecules that serve as dynamic signal modulators, versus the 
      more-recognized role of chronic modulation of signal transduction. In this study, 
      we investigated the role that fibronectin (FN) plays in the dynamic modulation of 
      transient receptor potential family V type 1 receptor (TRPV1) translocation to 
      the plasma membrane in trigeminal ganglia (TG) sensory neurons. Confocal 
      immunofluorescence analyses identify co-expression of the TRPV1 receptor with 
      integrin subunits that bind FN. TG neurons cultured upon or treated with FN 
      experienced a leftward shift in the EC(50) of capsaicin-stimulated neuropeptide 
      release. This FN-induced increase in TRPV1 sensitivity to activation is coupled 
      by an increase in plasma membrane expression of TRPV1, as well as an increase in 
      tyrosine phosphorylation of TRPV1 in TG neurons. Furthermore, TG neurons cultured 
      on FN demonstrated an increase in capsaicin-mediated Ca(2+) accumulation relative 
      to neurons cultured on poly-D-lysine. Data presented from these studies indicate 
      that FN stimulates tyrosine-phosphorylation-dependent translocation of the TRPV1 
      receptor to the plasma membrane, identifying FN as a critical component of the 
      ECM capable of sensory neuron sensitization.
FAU - Jeske, Nathaniel A
AU  - Jeske NA
AD  - Department of Oral and Maxillofacial Surgery, University of Texas Health Science 
      Center at San Antonio, San Antonio, Texas 78229-3900, USA. jeske@uthscsa.edu
FAU - Patwardhan, Amol M
AU  - Patwardhan AM
FAU - Henry, Michael A
AU  - Henry MA
FAU - Milam, Stephen B
AU  - Milam SB
LA  - eng
GR  - R01 DE015371/DE/NIDCR NIH HHS/United States
GR  - R01 DE015371-04/DE/NIDCR NIH HHS/United States
GR  - DE013942/DE/NIDCR NIH HHS/United States
GR  - R01 DE013942/DE/NIDCR NIH HHS/United States
GR  - DE015371/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20081111
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Fibronectins)
RN  - 0 (Integrins)
RN  - 0 (Neuropeptides)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (Trpv1 protein, rat)
RN  - 42HK56048U (Tyrosine)
RN  - 6SO6U10H04 (Biotin)
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
RN  - S07O44R1ZM (Capsaicin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Biotin/metabolism
MH  - Blotting, Western
MH  - Calcitonin Gene-Related Peptide/physiology
MH  - Calcium/metabolism
MH  - Capsaicin/pharmacology
MH  - Cells, Cultured
MH  - Extracellular Matrix/drug effects/metabolism
MH  - Fibronectins/*pharmacology
MH  - Immunohistochemistry
MH  - Integrins/metabolism
MH  - Male
MH  - Neuropeptides/metabolism
MH  - Phosphorylation
MH  - Protein Transport/*drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sensory Receptor Cells/*drug effects/*metabolism
MH  - Stimulation, Chemical
MH  - TRPV Cation Channels/*genetics/*metabolism
MH  - Trigeminal Ganglion/cytology/drug effects/metabolism
MH  - Tyrosine/metabolism
MH  - src-Family Kinases/physiology
PMC - PMC2678239
MID - NIHMS105683
EDAT- 2008/11/18 09:00
MHDA- 2009/02/25 09:00
PMCR- 2009/05/07
CRDT- 2008/11/18 09:00
PHST- 2008/11/18 09:00 [pubmed]
PHST- 2009/02/25 09:00 [medline]
PHST- 2008/11/18 09:00 [entrez]
PHST- 2009/05/07 00:00 [pmc-release]
AID - JNC5779 [pii]
AID - 10.1111/j.1471-4159.2008.05779.x [doi]
PST - ppublish
SO  - J Neurochem. 2009 Feb;108(3):591-600. doi: 10.1111/j.1471-4159.2008.05779.x. Epub 
      2008 Nov 11.