PMID- 19015004
OWN - NLM
STAT- MEDLINE
DCOM- 20090415
LR  - 20131121
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 158
IP  - 2
DP  - 2009 Jan 23
TI  - Neurogenesis inhibition in the dorsal vagal complex by chronic immobilization 
      stress in the adult rat.
PG  - 524-36
LID - 10.1016/j.neuroscience.2008.10.040 [doi]
AB  - The dorsal vagal complex (DVC) is the brainstem integrative center that mediates 
      the satiety reflex and relays autonomic neural responses to stress. The DVC 
      displays adult neurogenesis, intrinsic neural stem cells and a high brain-derived 
      neurotrophic factor (BDNF) content, effectors of plasticity that are modulated by 
      stress in the hippocampus. In this study we asked whether neurogenesis and BDNF 
      expression in the DVC are altered by stress, in parallel with food intake 
      reduction. To this end, neurogenesis was assessed in adult rats in vivo by 
      repetitive 5-bromo-2'-deoxyuridine (BrdU) administration without (controls) or 
      with daily sessions of immobilization stress (1 h/day), and were allowed to 
      survive for 2 weeks after the end of BrdU treatment. Neurogenic proliferation in 
      the brainstem was detected by immunohistochemistry and confocal microscopy mainly 
      in the area postrema and the nucleus tractus solitarius; newly formed neurons 
      amounted to about 35% of all BrdU-labeled cells in the DVC of control rats. 
      Chronic immobilization stress induced a significant decrease in neurogenic 
      proliferation in the DVC which reached 50% in the area postrema. The number of 
      newly-formed neurons was also decreased by chronic immobilization stress in the 
      DVC, and this effect was again maximal in the area postrema; the proportion of 
      BrdU-labeled cells that were neurons was unchanged. In vitro neurosphere assay 
      was then performed on microdissected DVC tissue from another cohort of 
      chronically stressed and control rats. Chronic immobilization stress induced a 
      significant decrease of the total neurosphere number per rat DVC in both primary 
      and secondary cultures, indicating that intrinsic neural stem cell frequency was 
      decreased by chronic stress in DVC tissue. Tissue BDNF concentration in the DVC, 
      as assessed by enzyme-linked immunosorbent assay, was not significantly altered 
      when compared with controls after 3, 6, 9 or 13 days of chronic immobilization 
      stress. These results further characterize neurogenesis in the DVC and suggest 
      its involvement in the long-term regulation of food intake.
FAU - Chigr, F
AU  - Chigr F
AD  - Lab. Biologie Fonctionnelle et Pathologique, Faculte des Sciences et Techniques, 
      BP 53, Beni-Mellal, Morocco.
FAU - Rachidi, F
AU  - Rachidi F
FAU - Segura, S
AU  - Segura S
FAU - Mahaut, S
AU  - Mahaut S
FAU - Tardivel, C
AU  - Tardivel C
FAU - Jean, A
AU  - Jean A
FAU - Najimi, M
AU  - Najimi M
FAU - Moyse, E
AU  - Moyse E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081030
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Area Postrema/*physiopathology
MH  - Body Weight/physiology
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Bromodeoxyuridine/metabolism
MH  - Cell Proliferation
MH  - Disease Models, Animal
MH  - Eating/physiology
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Male
MH  - Multivariate Analysis
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurogenesis/*physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Restraint, Physical/*methods
MH  - Solitary Nucleus/*physiopathology
MH  - Stress, Psychological/*pathology/physiopathology
MH  - Time Factors
EDAT- 2008/11/19 09:00
MHDA- 2009/04/16 09:00
CRDT- 2008/11/19 09:00
PHST- 2008/04/15 00:00 [received]
PHST- 2008/10/21 00:00 [revised]
PHST- 2008/11/19 00:00 [accepted]
PHST- 2008/11/19 09:00 [pubmed]
PHST- 2009/04/16 09:00 [medline]
PHST- 2008/11/19 09:00 [entrez]
AID - S0306-4522(08)01593-5 [pii]
AID - 10.1016/j.neuroscience.2008.10.040 [doi]
PST - ppublish
SO  - Neuroscience. 2009 Jan 23;158(2):524-36. doi: 10.1016/j.neuroscience.2008.10.040. 
      Epub 2008 Oct 30.