PMID- 1901525
OWN - NLM
STAT- MEDLINE
DCOM- 19910514
LR  - 20190510
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 12
IP  - 4
DP  - 1991 Apr
TI  - Dinitropyrene nitroreductase activity of purified NAD(P)H-quinone oxidoreductase: 
      role in rat liver cytosol and induction by Aroclor-1254 pretreatment.
PG  - 697-702
AB  - Dinitropyrenes (DNP) are potent bacterial mutagens in the Ames test and 
      genotoxins in cultured mammalian cells. Rat liver cytosol contains 
      nitroreductases that are critical in the activation of DNP to the ultimate 
      DNA-binding species. In order to study the nature and inducibility of liver 
      cytosolic enzymes involved in the activation of DNP, cytosolic nitroreductase 
      activities towards three DNP isomers (1,3-, 1.6- and 1,8-DNP) were determined in 
      Aroclor-pretreated and untreated rats. Aroclor-1254 pretreatment resulted in up 
      to 5-fold induction of cytosolic DNP nitroreductase. This induction was reflected 
      in at least a 15-fold increase in cytosolic NAD(P)H-quinone oxidoreductase (NQOR) 
      (E.C. 1.6.99.2) activity. The rates of nitroreduction for the three DNP isomers 
      followed the order 1,6- greater than 1,8- greater than 1,3-DNP in all cases 
      studied. 1,6-DNP nitroreductase coeluted with NQOR activity upon affinity 
      purification. Highly purified NQOR catalyzed the NADH- and NADPH-dependent 
      reduction of each of the three DNP isomers and displayed the same 
      stereospecificity as the cytosolic activity. These results provide evidence that 
      NQOR participates in the cytosolic nitroreduction of DNP and constitutes a major 
      part of the total DNP nitroreductase activity upon induction of NQOR by 
      Aroclor-1254 pretreatment. Thus, the role of NQOR in the metabolism of these 
      mutagens depends significantly upon the degree to which this enzyme is induced.
FAU - Hajos, A K
AU  - Hajos AK
AD  - Department of Biochemistry, Louisiana State University, Baton Rouge 70803.
FAU - Winston, G W
AU  - Winston GW
LA  - eng
GR  - AA06758-03/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (Aroclors)
RN  - 0 (Pyrenes)
RN  - 11097-69-1 (Chlorodiphenyl (54% Chlorine))
RN  - 53-59-8 (NADP)
RN  - EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone))
RN  - EC 1.6.99.- (Quinone Reductases)
RN  - EC 1.6.99.- (dinitropyrene nitroreductase)
RN  - EC 1.7.- (Nitroreductases)
SB  - IM
MH  - Animals
MH  - Aroclors/*pharmacology
MH  - Chlorodiphenyl (54% Chlorine)
MH  - Cytosol/enzymology
MH  - Enzyme Induction
MH  - Liver/enzymology
MH  - Male
MH  - NAD(P)H Dehydrogenase (Quinone)
MH  - NADP/metabolism
MH  - Nitroreductases/biosynthesis/*metabolism
MH  - Pyrenes/metabolism
MH  - Quinone Reductases/biosynthesis/*metabolism
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1991/04/01 00:00
MHDA- 1991/04/01 00:01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 1991/04/01 00:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
AID - 10.1093/carcin/12.4.697 [doi]
PST - ppublish
SO  - Carcinogenesis. 1991 Apr;12(4):697-702. doi: 10.1093/carcin/12.4.697.