PMID- 19016067
OWN - NLM
STAT- MEDLINE
DCOM- 20090501
LR  - 20191210
IS  - 1029-2330 (Electronic)
IS  - 1026-7158 (Linking)
VI  - 17
IP  - 1
DP  - 2009 Jan
TI  - Delivery of interleukin-18 gene to lung cancer cells using cationic emulsion.
PG  - 19-28
LID - 10.1080/10611860802438710 [doi]
AB  - Interleukin-18 (IL-18) is known to reduce melanoma lung metastases through 
      various mechanisms. For the delivery of IL-18 gene into the lung, three different 
      cationic emulsions as non-viral vectors were formulated using the same components 
      of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 
      1,2-dioleoyl-sn-glycero-3-trimethylammonium propane (DOTAP), and Tween 80 with 
      distinct oils. By using the small particle size of physicochemically stable E3, 
      the complex of E3/plasmid DNA encoding IL-18 (16:2.5, w/w) was transfected into 
      lung cancer cells, and the amount of plasmid DNA transferred and the expression 
      of both mRNA and protein for IL-18 were measured. When compared with 
      Lipofectamine/DNA complexes, an E3/DNA complex was less toxic and induced a 
      comparable cellular level of plasmid DNA and expression levels of both mRNA and 
      protein for IL-18. After injecting E3/DNA complexes into mice, the distribution 
      of plasmid DNA was the highest in the lung and the liver. Especially, the 
      administration of E3/DNA complexes induced a more rapid and prolonged 
      distribution of plasmid DNA encoding IL-18 into the lung than that of 
      Lipofectamine/DNA ones. These data demonstrated that cationic emulsion E3 
      containing castor oil could be useful for a delivery of IL-18 gene targeting the 
      lung as well as the liver without an additional homing device, implying a 
      potential IL-18 delivery system for the treatment of lung cancer.
FAU - Kang, Hyun-Suk
AU  - Kang HS
AD  - College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea.
FAU - Jin, Shun-Ji
AU  - Jin SJ
FAU - Myung, Chang-Seon
AU  - Myung CS
FAU - Hwang, Sung-Joo
AU  - Hwang SJ
FAU - Park, Jeong-Sook
AU  - Park JS
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Drug Target
JT  - Journal of drug targeting
JID - 9312476
RN  - 0 (1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine)
RN  - 0 (DNA Adducts)
RN  - 0 (Emulsifying Agents)
RN  - 0 (Emulsions)
RN  - 0 (Fatty Acids, Monounsaturated)
RN  - 0 (Interleukin-18)
RN  - 0 (Lipids)
RN  - 0 (Lipofectamine)
RN  - 0 (Olive Oil)
RN  - 0 (Phosphatidylethanolamines)
RN  - 0 (Plant Oils)
RN  - 0 (Polysorbates)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (RNA, Messenger)
RN  - 8001-22-7 (Soybean Oil)
RN  - 8001-79-4 (Castor Oil)
RN  - 9007-49-2 (DNA)
RN  - MR86K0XRQP (1,2-dioleoyloxy-3-(trimethylammonium)propane)
SB  - IM
MH  - Animals
MH  - Castor Oil/chemistry
MH  - Cell Line, Tumor
MH  - DNA/administration & dosage/chemistry/genetics/metabolism
MH  - DNA Adducts/administration & dosage/metabolism
MH  - Drug Stability
MH  - Emulsifying Agents/chemistry
MH  - Emulsions/*administration & dosage/*chemistry
MH  - Fatty Acids, Monounsaturated/chemistry
MH  - *Gene Transfer Techniques
MH  - Injections, Intravenous
MH  - Interleukin-18/*administration & dosage/*genetics
MH  - Lipids/administration & dosage
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Olive Oil
MH  - Phosphatidylethanolamines/chemistry
MH  - Plant Oils/chemistry
MH  - Plasmids/administration & dosage/chemistry/genetics/metabolism
MH  - Polysorbates/chemistry
MH  - Quaternary Ammonium Compounds/chemistry
MH  - RNA, Messenger/genetics/metabolism
MH  - Soybean Oil/chemistry
MH  - Swine
EDAT- 2008/11/19 09:00
MHDA- 2009/05/02 09:00
CRDT- 2008/11/19 09:00
PHST- 2008/11/19 09:00 [pubmed]
PHST- 2009/05/02 09:00 [medline]
PHST- 2008/11/19 09:00 [entrez]
AID - 905609103 [pii]
AID - 10.1080/10611860802438710 [doi]
PST - ppublish
SO  - J Drug Target. 2009 Jan;17(1):19-28. doi: 10.1080/10611860802438710.