PMID- 19021013
OWN - NLM
STAT- MEDLINE
DCOM- 20090219
LR  - 20090121
IS  - 1607-842X (Electronic)
IS  - 0891-6934 (Linking)
VI  - 42
IP  - 2
DP  - 2009 Feb
TI  - Chemoattractant mechanism of Th1 cells in class III and IV lupus nephritis.
PG  - 143-9
LID - 10.1080/08916930802438790 [doi]
AB  - Proliferative lupus nephritis (PLN) is the common, severe, and important form of 
      lupus nephritis. Recent report showed that T cells producing Interferon 
      (IFN)gamma (Th1 cells) increased in patients with World Health Organization class 
      IV. However, the relation between the increase of Th1 cells and the pathogenesis 
      has been made unclear. The aim of this study was to examine the chemoattractant 
      mechanism of Th1-producing cells and whether in vitro IFNgamma secretion from 
      Th1-producing cells in PLN. The Th1:Th2 ratio in peripheral blood was measured by 
      flow cytometry. The serum levels of IL-2, IFNgamma, IL-13, monocyte 
      chemoattractant protein-1 (MCP-1) and IP-10 were determined by using 
      enzyme-linked immunosorbent assay. In in vitro IFNgamma production assay, CD4(+)T 
      cells co-cultured with IL-12 and/or IL-18. Th1:Th2 ratio in PLN was high and not 
      correlated with the serum Th1 cytokine level. This Th1-producing cell tended to 
      go toward the inflammatory lesion by low CD62L expression and chemokines. The 
      level of MCP-1 and IP-10 in patients with PLN significantly increased. Lastly, in 
      vitro IFNgamma production assay, patients with PLN CD4(+)T cells produced 
      IFNgamma by the addition of IL-12 and IL-18, while CD4(+)T cells in normal 
      controls did not produce. These findings suggest that combination of Th1 inducers 
      and chemokine inhibition might be powerful threrapeutic approach in PLN.
FAU - Morimoto, Shinji
AU  - Morimoto S
AD  - Department of Internal Medicine and Rheumatology, Juntendo University School of 
      Medicine, Tokyo, Japan. morimoto@juntendo.ac.jp
FAU - Tokano, Yoshiaki
AU  - Tokano Y
FAU - Nakano, Souichiro
AU  - Nakano S
FAU - Watanabe, Takashi
AU  - Watanabe T
FAU - Tamayama, Yoshihiro
AU  - Tamayama Y
FAU - Mitsuo, Akiko
AU  - Mitsuo A
FAU - Suzuki, Jun
AU  - Suzuki J
FAU - Kaneko, Hiroshi
AU  - Kaneko H
FAU - Sekigawa, Iwao
AU  - Sekigawa I
FAU - Takasaki, Yoshinari
AU  - Takasaki Y
LA  - eng
PT  - Journal Article
PL  - England
TA  - Autoimmunity
JT  - Autoimmunity
JID - 8900070
RN  - 0 (CCL2 protein, human)
RN  - 0 (CXCL10 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokines)
RN  - 0 (IL2 protein, human)
RN  - 0 (Interleukin-13)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-2)
RN  - 0 (Interleukins)
RN  - 126880-86-2 (L-Selectin)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - CD4-Positive T-Lymphocytes/drug effects/immunology
MH  - Chemokine CCL2/blood
MH  - Chemokine CXCL10/blood
MH  - Chemokines/blood/*immunology
MH  - Female
MH  - Humans
MH  - Interferon-gamma/*immunology
MH  - Interleukin-12/pharmacology
MH  - Interleukin-13/blood
MH  - Interleukin-18/pharmacology
MH  - Interleukin-2/blood
MH  - Interleukins/blood/*immunology
MH  - L-Selectin/immunology/metabolism
MH  - Lupus Nephritis/blood/*immunology
MH  - Male
MH  - Middle Aged
MH  - Th1 Cells/*immunology/pathology
MH  - Th2 Cells/immunology
MH  - Young Adult
EDAT- 2008/11/21 09:00
MHDA- 2009/02/20 09:00
CRDT- 2008/11/21 09:00
PHST- 2008/11/21 09:00 [pubmed]
PHST- 2009/02/20 09:00 [medline]
PHST- 2008/11/21 09:00 [entrez]
AID - 905674529 [pii]
AID - 10.1080/08916930802438790 [doi]
PST - ppublish
SO  - Autoimmunity. 2009 Feb;42(2):143-9. doi: 10.1080/08916930802438790.