PMID- 19024307
OWN - NLM
STAT- MEDLINE
DCOM- 20090507
LR  - 20131121
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 39
IP  - 5
DP  - 2008 Sep
TI  - [The effect of NGF on hematopoiesis of granulocyte-macrophage in normal and 
      radiation, chemistry injury mice].
PG  - 757-62
AB  - OBJECTIVE: To explore the effect of nerve growth factor (NGF) on hematopoiesis of 
      granulocyte-macrophage and to elucidate the underlying mechanism. METHODS: Using 
      flow cytometry, colony forming assay, blood cell count, fluorescent real-time 
      quantitation PCR, and enzyme-linked immunosorbent assay (ELISA), changes in the 
      bone marrow cells (BMCs) proliferation cycle, CFU-GM counts, the peripheral white 
      blood cell (WBC)counts, BMC GM-CSF mRNA quantities, and serum GM-CSF, IL-3 
      concentrations were observed after NGF was injected intramuscularly into the 
      thigh of normal and combined 60Co gamma ray irradiated and cyclophosphamide 
      peritoneal cavity injected(combined radiation and chemistry injury mice, 60Co-Cy 
      injury) mice. The effect of NGF on CFU-GM formation in vitro cooperation with 
      exogenous GM-CSF or alone was also observed. RESULTS: An injection of NGF [7.5 
      microg/(kg x d) or 10 microg/(kg x d)] lasted 7 days, the S+G2/M proportions and 
      the CFU-GM counts of BMCs rose greater than injection of physiological saline 
      (PS) in normal and 60Co-Cy injury mice; the serum GM-CSF,IL-3 concentration in 
      60Co-Cy injury mice rose obviously. In vitro, NGF stimulated a dose-dependent 
      increase of CFU-GM colonies formation in the semisolid culture system with 
      exogenous rmGM-CSF. CONCLUSIONS: NGF cooperates with hematopoietic stimulating 
      factors in culture system to promote the CFU-GM colonies in normal and 60Co-Cy 
      injury mice in vitro. NGF accelerates the BMCs into mitosis, the hematopoietic 
      stem cells differentiation into granulocyte-macrophage hematopoietic cells, the 
      CFU-GM formation and promotes the white blood cells count in normal and 60Co-Cy 
      injury mice in vivo, also NGF stimulates the secreting of GM-CSF and IL-3 in 
      60Co-Cy injury mice,this may be one of the reasons why NGF stimulate the 
      granulocyte-macrophage hematopoiesis repair after combined radiation and 
      chemistry injury.
FAU - Huang, Xiao-qin
AU  - Huang XQ
AD  - Department of Histology, Embryology and Neurobiology, West China School of 
      Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
FAU - Zhu, Bi-de
AU  - Zhu BD
FAU - Jiang-Yang-ze-ren
AU  - Jiang-Yang-ze-ren
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Nerve Growth Factors)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - 8N3DW7272P (Cyclophosphamide)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/pathology
MH  - Cyclophosphamide/*adverse effects
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Granulocytes/physiology
MH  - Hematopoiesis/*drug effects/*radiation effects
MH  - Macrophages/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nerve Growth Factors/*pharmacology
MH  - Radiation Injuries/*blood
EDAT- 2008/11/26 09:00
MHDA- 2009/05/08 09:00
CRDT- 2008/11/26 09:00
PHST- 2008/11/26 09:00 [pubmed]
PHST- 2009/05/08 09:00 [medline]
PHST- 2008/11/26 09:00 [entrez]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Sep;39(5):757-62.