PMID- 19024536
OWN - NLM
STAT- MEDLINE
DCOM- 20090409
LR  - 20131121
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 88
IP  - 24
DP  - 2008 Jun 24
TI  - [Protective effects of inhibition of tissue nitric oxide in the initial stage 
      against tardive injury of testicular spermatogenic function: experiment with 
      rats].
PG  - 1676-8
AB  - OBJECTIVE: To study the protective effects of inhibition of tissue nitric oxide 
      in the initial stage against the tardive injury of contralateral testicular 
      spermatogenic function after unilateral testicular torsion. METHODS: 56 
      prepubertal Sprague-Dawley rats were randomly divided into 4 equal groups: 
      placebo group undergoing rotation of left testis 720 degrees clockwise for 4 h, 
      fixing thereof to the scrotum, and then intravenous injection of normal saline; 
      cyclosporine group, undergoing intraperitoneal injection of cyclosporine once 
      daily for 1 month after the testicular rotation and fixation; NG-methyl 
      L-arginine (L-NMMA) group, undergoing intravenous injection of L-NMMA 30 mg/kg 30 
      min before the testicular rotation; and sham-operation group, undergoing 
      isolation and suture of testis only. The right (untwisted) testes were removed 
      from 7 rats from each group 1 week and 2 months after surgery. The 
      malondialdehyde (MDA) level and nitrous oxide (NO)/nitrogen oxide synthase (NOS) 
      content were evaluated. Histological examination was conducted. The mean 
      seminiferous tubule diameter (MSTD) was assessed. The level of MHC 
      peptide-tetramer complex was determined by flow cytometry. RESULTS: The levels of 
      MDA, NO, NOS, and MHC peptide-tetramer complex of the L-NMMA, placebo, and 
      cyclosporine groups one week after surgery were all significantly higher than 
      those of the sham operation group (all P < 0.05). The levels of MDA, NO, NOS, and 
      MHC peptide-tetramer complex of the L-NMMA group were all significantly lower 
      then those of the placebo group (all P < 0.05). The pathological damage of the 
      contralateral testis in early and late stages in the L-NMMA group was lighter 
      than in the placebo group. CONCLUSION: By inhibiting tissue NO production in 
      focal organizations during the early period, L-NMMA reduces the damages inthe 
      testis contralateral to the testis undergoing unilateral torsion.
FAU - Lin, Xiao-kun
AU  - Lin XK
AD  - Department of Pediatric Surgery, Affiliated Yuying Children's Hospital of Wenzhou 
      Medical College, Wenzhou 325027, China.
FAU - Qin, Le
AU  - Qin L
FAU - Chen, Cong-de
AU  - Chen CD
FAU - Han, Yi-xiang
AU  - Han YX
FAU - Chen, Xiao-ming
AU  - Chen XM
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Enzyme Inhibitors)
RN  - 27JT06E6GR (omega-N-Methylarginine)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
SB  - IM
MH  - Animals
MH  - Enzyme Inhibitors/pharmacology
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Nitric Oxide/*antagonists & inhibitors/metabolism
MH  - Nitric Oxide Synthase/antagonists & inhibitors/metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seminiferous Tubules/*drug effects/metabolism/physiopathology
MH  - Spermatic Cord Torsion/metabolism/physiopathology/*prevention & control
MH  - omega-N-Methylarginine/*pharmacology
EDAT- 2008/11/26 09:00
MHDA- 2009/04/10 09:00
CRDT- 2008/11/26 09:00
PHST- 2008/11/26 09:00 [pubmed]
PHST- 2009/04/10 09:00 [medline]
PHST- 2008/11/26 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2008 Jun 24;88(24):1676-8.