PMID- 19026760
OWN - NLM
STAT- MEDLINE
DCOM- 20090505
LR  - 20161020
IS  - 1286-4579 (Print)
IS  - 1286-4579 (Linking)
VI  - 11
IP  - 1
DP  - 2009 Jan
TI  - Peptides from Paracoccidioides brasiliensis GP43 inhibit macrophage functions and 
      inflammatory response.
PG  - 92-9
LID - 10.1016/j.micinf.2008.10.011 [doi]
AB  - Paracoccidioidomycosis (PCM) is a systemic granulomatous disease caused by 
      Paracoccidioides brasiliensis (Pb), a thermal dimorphic fungus. Its major antigen 
      is a 43-kDa glycoprotein. Gp43 embodies different functions: it participates in 
      evasion mechanisms during the installation of primary infection, stimulates 
      granuloma-like formation in vitro and presents T-cell epitopes that induce 
      protective response against the fungus. Here, we investigated epitopes from gp43 
      inhibitory of both, macrophage functions and inflammatory reaction. Different 
      gp43 peptides, spanning the entire sequence of the molecule, were added to 
      cultures of bone marrow-derived macrophages. After challenge with zymosan or Pb 
      cells, phagocytic indexes were measured. Peptides expressed on the molecule 
      surface were determined by graphic analysis using the Protean module; DNAstar 
      Inc. Two peptides which decreased phagocytic index and were expressed at the 
      surface of the molecule, P4 and P23, were selected for further studies. It was 
      shown that both inhibited the release of NO by zymosan stimulated macrophages 
      while enhanced release of H(2)O(2). The release of TNF-alpha in culture 
      supernatants from in vitro phagocytic tests showed different response depending 
      of P4 concentration (data not shown). In vivo assays with Mycobacterium bovis - 
      bacillus Calmette-Guerin (BCG) or Pb cells demonstrated that these peptides 
      presented non-specific and specific anti-inflammatory properties.
FAU - Konno, Adriana Y C
AU  - Konno AY
AD  - Universidade Federal de Sao Paulo - UNIFESP, Department of Microbiology, 
      Immunology and Parasitology, Discipline of Immunology, Sao Paulo, Brazil.
FAU - Maricato, Juliana T
AU  - Maricato JT
FAU - Konno, Fabiana T C
AU  - Konno FT
FAU - Mariano, Mario
AU  - Mariano M
FAU - Lopes, Jose Daniel
AU  - Lopes JD
LA  - eng
PT  - Journal Article
DEP - 20081106
PL  - France
TA  - Microbes Infect
JT  - Microbes and infection
JID - 100883508
RN  - 0 (43 kDa protein, Paracoccidioides)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Fungal Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Peptides)
SB  - IM
MH  - Animals
MH  - Antigens, Fungal/*chemistry/pharmacology
MH  - Cells, Cultured
MH  - Female
MH  - Fungal Proteins/*chemistry/pharmacology
MH  - Glycoproteins/*chemistry/pharmacology
MH  - Inflammation/drug therapy
MH  - Macrophages/*drug effects/immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Paracoccidioides/chemistry/*immunology
MH  - Paracoccidioidomycosis/microbiology
MH  - Peptides/chemical synthesis/chemistry/*pharmacology
MH  - Phagocytosis/drug effects
EDAT- 2008/11/26 09:00
MHDA- 2009/05/06 09:00
CRDT- 2008/11/26 09:00
PHST- 2008/10/09 00:00 [received]
PHST- 2008/10/20 00:00 [accepted]
PHST- 2008/11/26 09:00 [pubmed]
PHST- 2009/05/06 09:00 [medline]
PHST- 2008/11/26 09:00 [entrez]
AID - S1286-4579(08)00303-1 [pii]
AID - 10.1016/j.micinf.2008.10.011 [doi]
PST - ppublish
SO  - Microbes Infect. 2009 Jan;11(1):92-9. doi: 10.1016/j.micinf.2008.10.011. Epub 
      2008 Nov 6.