PMID- 19027805
OWN - NLM
STAT- MEDLINE
DCOM- 20090519
LR  - 20211020
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 140
IP  - 1-2
DP  - 2009 Mar
TI  - Analysis of Foot-and-mouth disease virus nucleotide sequence variation within 
      naturally infected epithelium.
PG  - 199-204
LID - 10.1016/j.virusres.2008.10.012 [doi]
AB  - An important epidemiological tool in the control of epidemics of Foot-and-mouth 
      disease (FMD) is genetic tracing using complete virus genome sequence data. 
      However to interpret these genetic data, it is important to quantify underlying 
      variation present in FMDV populations from individual tissue samples. Cloned 
      complete capsid sequences from two virus populations from epithelium from a cow 
      (n=26) and from a sheep (n=15) infected during the UK 2001 outbreaks were 
      generated. Genetic diversity of the two virus populations differed significantly, 
      with sequences representing virus from the cow having a mutation frequency of 
      2.79 x 10(-4) mutations per nucleotide sequenced (mpns) and those from the sheep 
      having 3.94 x 10(-4) mpns (chi(2)=8.24, P=0.004). The dN/dS ratio of sequences 
      from the cow was higher (1.228) than that from the sheep (0.187) although not 
      significantly so. The sequences from the cow epithelium exhibited significantly 
      higher than expected number of changes within neutralising antigenic sites 
      (P=0.0007). The performance of two different reverse transcriptase enzymes was 
      found not to differ with respect to the frequency (P=0.559, chi(2)=0.341) or 
      dN/dS ratio (P=0.863, chi(2)=0.03) of the mutations observed. These data provide 
      insight into the population diversity that exists within a single lesion and help 
      toward understanding the mechanisms that underpin sequence evolution of FMDV.
FAU - Cottam, Eleanor M
AU  - Cottam EM
AD  - Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, 
      Surrey, England GU24 0NF, United Kingdom. E.Cottam@uea.ac.uk
FAU - King, Donald P
AU  - King DP
FAU - Wilson, Anthony
AU  - Wilson A
FAU - Paton, David J
AU  - Paton DJ
FAU - Haydon, Daniel T
AU  - Haydon DT
LA  - eng
GR  - BB/F005733/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BBS/E/I/00001409/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081212
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Capsid Proteins)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Capsid Proteins/genetics
MH  - Cattle/virology
MH  - Cattle Diseases/epidemiology/virology
MH  - Consensus Sequence
MH  - Disease Outbreaks/veterinary
MH  - Epithelium/*virology
MH  - Evolution, Molecular
MH  - Foot-and-Mouth Disease/epidemiology/*virology
MH  - Foot-and-Mouth Disease Virus/*genetics
MH  - Genetic Variation
MH  - Genome, Viral
MH  - Models, Genetic
MH  - Molecular Epidemiology
MH  - Mutation
MH  - RNA, Viral/genetics
MH  - Sequence Analysis, RNA
MH  - Sheep/virology
MH  - Sheep Diseases/epidemiology/virology
EDAT- 2008/11/26 09:00
MHDA- 2009/05/20 09:00
CRDT- 2008/11/26 09:00
PHST- 2008/06/12 00:00 [received]
PHST- 2008/10/23 00:00 [revised]
PHST- 2008/10/28 00:00 [accepted]
PHST- 2008/11/26 09:00 [entrez]
PHST- 2008/11/26 09:00 [pubmed]
PHST- 2009/05/20 09:00 [medline]
AID - S0168-1702(08)00384-5 [pii]
AID - 10.1016/j.virusres.2008.10.012 [doi]
PST - ppublish
SO  - Virus Res. 2009 Mar;140(1-2):199-204. doi: 10.1016/j.virusres.2008.10.012. Epub 
      2008 Dec 12.