PMID- 19027988
OWN - NLM
STAT- MEDLINE
DCOM- 20090622
LR  - 20200930
IS  - 1618-1328 (Electronic)
IS  - 0176-1617 (Linking)
VI  - 166
IP  - 6
DP  - 2009 Apr 1
TI  - Chlorophenols induce lipid peroxidation and change antioxidant parameters in the 
      leaves of wheat (Triticum aestivum L.).
PG  - 559-68
LID - 10.1016/j.jplph.2008.09.011 [doi]
AB  - In this work, changes in superoxide dismutase (SOD), catalase (CAT) and guaiacol 
      peroxidase (POD) activity were determined in the leaves of wheat (Triticum 
      aestivum L.) exposed to 2,4-dichlorophenol (2,4-DCP) and pentachlorophenol (PCP). 
      We analyzed the content of free phenols, the level of lipid peroxidation, and 
      also the oxidation of dihydrorhodamine 123 by 2,4-DCP and PCP. Chlorophenols were 
      spiked to soil in concentrations of 0.5 and 5.0 mg kg(-1). Plant seeds were 
      raised in plastic pots containing soil at a temperature of 25 degrees C with a 
      16-h photoperiod and irradiance of 250 micromol m(-2) s(-1). The leaves were 
      harvested on the third, sixth and twelfth days of the experiment. The inhibition 
      of SOD activity in the leaves of wheat was observed for 2,4-DCP and PCP. 2,4-DCP 
      and PCP induced changes in CAT activity with a stronger effect for PCP. The 
      compounds markedly increased guaiacol POD activity during 12d of the exposition 
      of wheat to their action. The increase in free phenol content was observed both 
      for 2,4-DCP and PCP. Chlorophenols also induced a powerful lipid peroxidation 
      process between the third and sixth days of the experiment. A higher 
      concentration of chlorophenols used in our study induced greater changes in all 
      of the investigated parameters. 2,4-DCP and PCP oxidized the fluorescent probe - 
      dihydrorhodamine 123 - in the concentrations of 5 and 1 ppm, respectively, and 
      the addition of magnesium ions enhanced the oxidative capacity of the examined 
      xenobiotics.
FAU - Michalowicz, Jaromir
AU  - Michalowicz J
AD  - Department of Environmental Pollution Biophysics, Lodz, Poland. 
      michalow@biol.uni.lodz.pl
FAU - Posmyk, Malgorzata
AU  - Posmyk M
FAU - Duda, Wirgiliusz
AU  - Duda W
LA  - eng
PT  - Journal Article
DEP - 20081122
PL  - Germany
TA  - J Plant Physiol
JT  - Journal of plant physiology
JID - 9882059
RN  - 0 (Antioxidants)
RN  - 0 (Chlorophenols)
RN  - D9BSU0SE4T (Pentachlorophenol)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Analysis of Variance
MH  - Antioxidants/*metabolism
MH  - Catalase/metabolism
MH  - Chlorophenols/*pharmacology
MH  - Lipid Peroxidation/*drug effects
MH  - Oxidation-Reduction
MH  - Pentachlorophenol/*pharmacology
MH  - Plant Leaves/*drug effects/enzymology/*metabolism
MH  - Superoxide Dismutase/metabolism
MH  - Triticum/drug effects/enzymology/*metabolism
EDAT- 2008/11/26 09:00
MHDA- 2009/06/23 09:00
CRDT- 2008/11/26 09:00
PHST- 2008/02/21 00:00 [received]
PHST- 2008/08/12 00:00 [revised]
PHST- 2008/09/04 00:00 [accepted]
PHST- 2008/11/26 09:00 [pubmed]
PHST- 2009/06/23 09:00 [medline]
PHST- 2008/11/26 09:00 [entrez]
AID - S0176-1617(08)00291-5 [pii]
AID - 10.1016/j.jplph.2008.09.011 [doi]
PST - ppublish
SO  - J Plant Physiol. 2009 Apr 1;166(6):559-68. doi: 10.1016/j.jplph.2008.09.011. Epub 
      2008 Nov 22.