PMID- 19028311
OWN - NLM
STAT- MEDLINE
DCOM- 20090306
LR  - 20081125
IS  - 1095-9998 (Electronic)
IS  - 0740-0020 (Linking)
VI  - 26
IP  - 1
DP  - 2009 Feb
TI  - Kinetics of resuscitation and growth of L. monocytogenes as a tool to select 
      appropriate enrichment conditions as a prior step to rapid detection methods.
PG  - 88-93
LID - 10.1016/j.fm.2008.08.007 [doi]
AB  - Rapid methods still rely on a prior (shortened) enrichment step before 
      application. Quantitative information is a prerequisite for understanding the 
      resuscitation kinetics of the growth during the enrichment step. In this study 
      various basal and newly introduced selective enrichment broths were evaluated. 
      First, growth parameters (lambda, mu(max)) of both healthy and sub-lethally 
      injured cells were determined. Next, a selection of enrichment broths was 
      compared for their capacity to support detection within 24h of low numbers of 
      Listeria monocytogenes in artificially and naturally contaminated food samples. 
      Detection was performed either by phage protein-based capture (Listeria Capture 
      kit, Profos, Regensburg, Germany) combined with plating on chromogenic medium or 
      by fluorescence in situ hybridization (FISH) using the VIT-Listeria kit 
      (Vermicon, Munich, Germany). Kinetics of resuscitation and growth of L. 
      monocytogenes in various enrichment broths showed that for detection of low 
      numbers of sub-lethally injured L. monocytogenes cells at least an overnight 
      enrichment was needed. A selective enrichment broth was needed to enable 
      proliferation of L. monocytogenes within the indigenous bacterial flora present 
      in foods. However, combination of an appropriate enrichment condition with 
      advanced detection techniques may enable a 24h detection of L. monocytogenes.
FAU - Jasson, Vicky
AU  - Jasson V
AD  - Laboratory of Food Microbiology and Food Preservation, Department of Food Safety 
      and Food Quality, Faculty of Bioscience Engineering, Ghent University, Coupure 
      links 653, B-9000 Ghent, Belgium.
FAU - Rajkovic, Andreja
AU  - Rajkovic A
FAU - Debevere, Johan
AU  - Debevere J
FAU - Uyttendaele, Mieke
AU  - Uyttendaele M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080903
PL  - England
TA  - Food Microbiol
JT  - Food microbiology
JID - 8601127
RN  - 0 (Culture Media)
SB  - IM
MH  - Colony Count, Microbial/methods
MH  - Consumer Product Safety
MH  - Culture Media/*chemistry
MH  - Food Contamination/*analysis
MH  - Food Microbiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Kinetics
MH  - Listeria monocytogenes/*growth & development/isolation & purification
MH  - *Models, Biological
MH  - Sensitivity and Specificity
MH  - Time Factors
EDAT- 2008/11/26 09:00
MHDA- 2009/03/07 09:00
CRDT- 2008/11/26 09:00
PHST- 2008/05/27 00:00 [received]
PHST- 2008/08/21 00:00 [revised]
PHST- 2008/08/23 00:00 [accepted]
PHST- 2008/11/26 09:00 [pubmed]
PHST- 2009/03/07 09:00 [medline]
PHST- 2008/11/26 09:00 [entrez]
AID - S0740-0020(08)00172-X [pii]
AID - 10.1016/j.fm.2008.08.007 [doi]
PST - ppublish
SO  - Food Microbiol. 2009 Feb;26(1):88-93. doi: 10.1016/j.fm.2008.08.007. Epub 2008 
      Sep 3.