PMID- 19028995
OWN - NLM
STAT- MEDLINE
DCOM- 20090210
LR  - 20211020
IS  - 1535-9786 (Electronic)
IS  - 1535-9778 (Print)
IS  - 1535-9786 (Linking)
VI  - 8
IP  - 1
DP  - 2009 Jan
TI  - Endocytosis is crucial for cell polarity and apical membrane recycling in the 
      filamentous fungus Aspergillus oryzae.
PG  - 37-46
LID - 10.1128/EC.00207-08 [doi]
AB  - Establishing the occurrence of endocytosis in filamentous fungi was elusive in 
      the past mainly due to the lack of reliable indicators of endocytosis. Recently, 
      however, it was shown that the fluorescent dye 
      N-(3-triethylammoniumpropyl)-4-(p-diethyl-aminophenyl-hexatrienyl)pyridinium 
      dibromide (FM4-64) and the plasma membrane protein AoUapC (Aspergillus oryzae 
      UapC) fused to enhanced green fluorescent protein (EGFP) were internalized from 
      the plasma membrane by endocytosis. Although the occurrence of endocytosis was 
      clearly demonstrated, its physiological importance in filamentous fungi still 
      remains largely unaddressed. We generated a strain in which A. oryzae end4 
      (Aoend4), the A. oryzae homolog of Saccharomyces cerevisiae END4/SLA2, was 
      expressed from the Aoend4 locus under the control of a regulatable thiA promoter. 
      The growth of this strain was severely impaired, and its hyphal morphology was 
      altered in the Aoend4-repressed condition. Moreover, in the Aoend4-repressed 
      condition, neither FM4-64 nor AoUapC-EGFP was internalized, indicating defective 
      endocytosis. Furthermore, the localization of a secretory soluble 
      N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) was 
      abnormal in the Aoend4-repressed condition. Aberrant accumulation of cell wall 
      components was also observed by calcofluor white staining and transmission 
      electron microscopy analysis, and several genes that encode cell wall-building 
      enzymes were upregulated, indicating that the regulation of cell wall synthesis 
      is abnormal in the Aoend4-repressed condition, whereas Aopil1 disruptants do not 
      display the phenotype exhibited in the Aoend4-repressed condition. Our results 
      strongly suggest that endocytosis is crucial for the hyphal tip growth in 
      filamentous fungi.
FAU - Higuchi, Yujiro
AU  - Higuchi Y
AD  - Department of Biotechnology, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, 
      Japan.
FAU - Shoji, Jun-ya
AU  - Shoji JY
FAU - Arioka, Manabu
AU  - Arioka M
FAU - Kitamoto, Katsuhiko
AU  - Kitamoto K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081121
PL  - United States
TA  - Eukaryot Cell
JT  - Eukaryotic cell
JID - 101130731
RN  - 0 (Fungal Proteins)
SB  - IM
MH  - Aspergillus oryzae/*cytology/genetics/metabolism
MH  - Cell Membrane/genetics/*metabolism
MH  - Cell Polarity
MH  - Cell Wall/genetics/metabolism
MH  - *Endocytosis
MH  - Fungal Proteins/genetics/metabolism
MH  - Genetic Complementation Test
PMC - PMC2620746
EDAT- 2008/11/26 09:00
MHDA- 2009/02/12 09:00
PMCR- 2009/07/01
CRDT- 2008/11/26 09:00
PHST- 2008/11/26 09:00 [pubmed]
PHST- 2009/02/12 09:00 [medline]
PHST- 2008/11/26 09:00 [entrez]
PHST- 2009/07/01 00:00 [pmc-release]
AID - EC.00207-08 [pii]
AID - 0207-08 [pii]
AID - 10.1128/EC.00207-08 [doi]
PST - ppublish
SO  - Eukaryot Cell. 2009 Jan;8(1):37-46. doi: 10.1128/EC.00207-08. Epub 2008 Nov 21.