PMID- 19029406
OWN - NLM
STAT- MEDLINE
DCOM- 20090323
LR  - 20211020
IS  - 0022-1554 (Print)
IS  - 1551-5044 (Electronic)
IS  - 0022-1554 (Linking)
VI  - 57
IP  - 3
DP  - 2009 Mar
TI  - Differential localizations of the transient receptor potential channels TRPV4 and 
      TRPV1 in the mouse urinary bladder.
PG  - 277-87
LID - 10.1369/jhc.2008.951962 [doi]
AB  - We studied the localization and physiological functions of the transient receptor 
      potential (TRP) channels TRPV1 (TRP vanilloid 1) and TRPV4 (TRP vanilloid 4) in 
      the mouse bladder, because both channels are thought to be mechanosensors for 
      bladder distention. RT-PCR specifically amplified TRPV4 transcripts from the 
      urothelial cells, whereas TRPV1 transcripts were barely detectable. ISH 
      experiments showed that TRPV4 transcripts were abundantly expressed in the 
      urothelium, whereas TRPV1 transcripts were not detectable in the urothelial 
      cells. Immunoblotting and IHC studies showed that TRPV4 proteins were mainly 
      localized at the basal plasma membrane domains of the basal urothelial cells. In 
      contrast, TRPV1-immunoreactivities were found not in the urothelial cells but in 
      the nerve fibers that innervate the urinary bladder. In Ca(2+)-imaging 
      experiments, 4alpha-phorbol 12,13-didecanoate, a TRPV4 agonist, and hypotonic 
      stimuli induced significant increases in intracellular calcium ion concentration 
      ([Ca(2+)](i)) in isolated urothelial cells, whereas capsaicin, a TRPV1 agonist, 
      showed no marked effect on the cells. These findings raise the possibility that, 
      in mouse urothelial cells, TRPV4 may contribute to the detection of increases in 
      intravesical pressure related to the micturition reflex.
FAU - Yamada, Takahiro
AU  - Yamada T
AD  - Department of Neurobiology and Anatomy, Graduate School of Medical Sciences, 
      Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 
      467-8601, Japan.
FAU - Ugawa, Shinya
AU  - Ugawa S
FAU - Ueda, Takashi
AU  - Ueda T
FAU - Ishida, Yusuke
AU  - Ishida Y
FAU - Kajita, Kenji
AU  - Kajita K
FAU - Shimada, Shoichi
AU  - Shimada S
LA  - eng
PT  - Journal Article
DEP - 20081124
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (4alpha-phorbol 12,13-didecanone)
RN  - 0 (Phorbols)
RN  - 0 (RNA, Messenger)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (TRPV1 protein, mouse)
RN  - 0 (Trpv4 protein, mouse)
RN  - S07O44R1ZM (Capsaicin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/metabolism
MH  - Capsaicin/pharmacology
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Microscopy, Immunoelectron
MH  - Nerve Fibers/metabolism
MH  - Phorbols/pharmacology
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - TRPV Cation Channels/agonists/genetics/*metabolism
MH  - Urinary Bladder/cytology/innervation/*metabolism
MH  - Urothelium/cytology/innervation/metabolism
PMC - PMC2664935
EDAT- 2008/11/26 09:00
MHDA- 2009/03/24 09:00
PMCR- 2010/03/01
CRDT- 2008/11/26 09:00
PHST- 2008/11/26 09:00 [pubmed]
PHST- 2009/03/24 09:00 [medline]
PHST- 2008/11/26 09:00 [entrez]
PHST- 2010/03/01 00:00 [pmc-release]
AID - jhc.2008.951962 [pii]
AID - 951962 [pii]
AID - 10.1369/jhc.2008.951962 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2009 Mar;57(3):277-87. doi: 10.1369/jhc.2008.951962. Epub 
      2008 Nov 24.