PMID- 19030026
OWN - NLM
STAT- MEDLINE
DCOM- 20081222
LR  - 20210102
IS  - 1748-7838 (Electronic)
IS  - 1001-0602 (Linking)
VI  - 18
IP  - 12
DP  - 2008 Dec
TI  - The role of granulocyte macrophage-colony-stimulating factor in acute intestinal 
      inflammation.
PG  - 1220-9
LID - 10.1038/cr.2008.310 [doi]
AB  - An imbalance of mucosal pro- and anti-inflammatory cytokines is crucial in the 
      pathogenesis of inflammatory bowel disease (IBD). GM-CSF influences the 
      development of hemopoietic cells. The precise role of GM-CSF in IBD remains to be 
      elucidated. GM-CSF gene knockout (GM-CSF(-/-)) and wild-type (Wt) mice were 
      challenged with 2.5% dextran sulfate sodium (DSS) for 7 days. The ensued clinical 
      and pathological changes, macrophage infiltration, colonic cytokine production, 
      and bacterial counts were examined. DSS-treated GM-CSF(-/-) mice developed more 
      severe acute colitis than DSS-treated Wt mice, reflected by a greater body weight 
      loss, more rectal bleeding, and aggravated histopathological changes. More 
      infiltrating macrophages were observed in GM-CSF(-/-), compared with Wt mice 
      following DSS challenge, correlating with monocyte chemoattractant protein-1 
      (MCP-1) production. The levels of colonic IL-17 and TNF-alpha were increased 
      significantly in GM-CSF(-/-) mice, but not in Wt mice, following DSS 
      administration. The level of IL-6 was increased by 1.5- and 2-fold in the colon 
      of GM-CSF(-/-) and Wt mice, respectively, following DSS challenge. No significant 
      changes in IL-4 and IFN-gamma were detected in Wt and GM-CSF(-/-) mice following 
      DSS treatment. The bacteria recovery from colon was increased about 15- and 
      5-fold, respectively, in Wt mice and GM-CSF(-/-) mice following DSS challenge. 
      These results suggest that GM-CSF(-/-) mice are more susceptible to acute 
      DSS-induced colitis, possibly because of an impaired gut innate immune response 
      as a result of diminished GM-CSF.
FAU - Xu, Yinghua
AU  - Xu Y
AD  - Department of Pathology (D06), Bosch Institute, School of Medical Sciences, 
      University of Sydney, New South Wales 2006, Australia.
FAU - Hunt, Nicholas H
AU  - Hunt NH
FAU - Bao, Shisan
AU  - Bao S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Res
JT  - Cell research
JID - 9425763
RN  - 0 (Cytokines)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - 9042-14-2 (Dextran Sulfate)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Colitis/*immunology/pathology
MH  - Cytokines/immunology
MH  - Dextran Sulfate
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*deficiency/*immunology
MH  - Immunohistochemistry
MH  - Inflammation/pathology
MH  - Inflammatory Bowel Diseases/*immunology/pathology
MH  - Intestines/microbiology/pathology
MH  - Mice
MH  - Mice, Knockout
EDAT- 2008/11/26 09:00
MHDA- 2008/12/23 09:00
CRDT- 2008/11/26 09:00
PHST- 2008/11/26 09:00 [pubmed]
PHST- 2008/12/23 09:00 [medline]
PHST- 2008/11/26 09:00 [entrez]
AID - cr2008310 [pii]
AID - 10.1038/cr.2008.310 [doi]
PST - ppublish
SO  - Cell Res. 2008 Dec;18(12):1220-9. doi: 10.1038/cr.2008.310.