PMID- 19030709
OWN - NLM
STAT- MEDLINE
DCOM- 20090707
LR  - 20190606
IS  - 1414-431X (Electronic)
IS  - 0100-879X (Linking)
VI  - 41
IP  - 10
DP  - 2008 Oct
TI  - Estrogen receptor 1 gene polymorphisms in premenopausal women: interaction 
      between genotype and smoking on lipid levels.
PG  - 872-6
LID - S0100-879X2008001000007 [pii]
AB  - Estrogen has multiple effects on lipid and lipoprotein metabolism. We 
      investigated the association between the four common single nucleotide 
      polymorphisms in the estrogen receptor 1 (ESR1) gene locus, -1989T>G, +261G>C, 
      IVS1-397T>C and IVS1-351A>G, and lipid and lipoprotein levels in southern 
      Brazilians. The sample consisted in 150 men and 187 premenopausal women. The 
      women were considered premenopausal if they had regular menstrual bleeding within 
      the previous 3 months and were 18-50 years of age. Exclusion criteria were 
      pregnancy, secondary hyperlipidemia due to renal, hepatic or thyroid disease, and 
      diabetes. Smoking status was self-reported; subjects were classified as never 
      smoked and current smokers. DNA was amplified by PCR and was subsequently 
      digested with the appropriate restriction enzymes. Statistical analysis was 
      carried out for men and women separately. In the study population, major allele 
      frequencies were _1989*T (0.83), +261*G (0.96), IVS1-397*T (0.58), and IVS1-351*A 
      (0.65). Multiple linear regression analyses indicated that an interaction between 
      +261G>C polymorphism and smoking was a significant factor affecting high-density 
      lipoprotein cholesterol (HDL-C) levels (P = 0.028) in women. Nonsmoking women 
      with genotype G/C of +261G>C polymorphism had mean HDL-C levels higher than those 
      with G/G genotype (1.40 +/- 0.33 vs 1.22 +/- 0.26 mmol/L; P = 0.033). No 
      significant associations with lipid and lipoprotein levels in women and men were 
      detected for other polymorphisms. In conclusion, the +261G>C polymorphism might 
      influence lipoprotein and lipid levels in premenopausal women, but these effects 
      seem to be modulated by smoking, whereas in men ESR1 polymorphisms were not 
      associated with high lipoprotein levels.
FAU - Almeida, S
AU  - Almeida S
AD  - Departamento de Genetica, Instituto de Biociencias, Universidade Federal do Rio 
      Grande do Sul, Porto Alegre, RS, Brasil.
FAU - Hutz, M H
AU  - Hutz MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Lipids)
SB  - IM
MH  - Adult
MH  - Estrogen Receptor alpha/*genetics
MH  - Female
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Humans
MH  - Lipids/*blood
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Single Nucleotide/*genetics
MH  - Premenopause/blood/*genetics
MH  - Smoking/blood/*genetics
MH  - Young Adult
EDAT- 2008/11/26 09:00
MHDA- 2009/07/08 09:00
CRDT- 2008/11/26 09:00
PHST- 2008/04/22 00:00 [received]
PHST- 2008/10/21 00:00 [accepted]
PHST- 2008/11/26 09:00 [pubmed]
PHST- 2009/07/08 09:00 [medline]
PHST- 2008/11/26 09:00 [entrez]
AID - S0100-879X2008001000007 [pii]
AID - 10.1590/s0100-879x2008001000007 [doi]
PST - ppublish
SO  - Braz J Med Biol Res. 2008 Oct;41(10):872-6. doi: 10.1590/s0100-879x2008001000007.