PMID- 19031715 OWN - NLM STAT- MEDLINE DCOM- 20100928 LR - 20160818 IS - 0529-5807 (Print) IS - 0529-5807 (Linking) VI - 37 IP - 6 DP - 2008 Jun TI - [Clinicopathologic significance of bcl-6 gene rearrangement and expression in three molecular subgroups of diffuse large B-cell lymphoma]. PG - 371-6 AB - OBJECTIVE: To investigate the role of bcl-6 gene rearrangement and bcl-6 expression in three molecular subgroups of diffuse large B-cell lymphoma (DLBCL) and its clinicopathological significance. METHODS: Tissue microarray including 163 newly diagnosed DLBCL was constructed. Fluorescence in situ hybridization (FISH) was performed to detect the bcl-6 gene rearrangement and immunohistochemistry (EnVision method) was used to evaluate the expression of bcl-6, Ki-67, cyclin D3, Geminin and P27(Kip1) proteins in DLBCL. The association with clinicopathological features was analyzed. RESULTS: One hundred and forty nine of 163 cases were further classified into three molecular subgroups: 40 cases of germinal center B-cell-like (GCB) type, 75 cases of activated non-germinal center B-cell-like (ABC) type, 34 cases of Type 3. Of these 149 cases, FISH for bcl-6 gene rearrangement was successful in 118 cases. bcl-6 gene rearrangement was observed in 33 of 118 (28.0%) cases. The bcl-6 gene rearrangement was more frequently seen in the ABC subgroup (22/62, 35.5%) than in GCB (6/31, 19.4%) and Type 3 subgroups (5/25, 20.0%, P=0.16). The correlation of bcl-6 gene rearrangement and expression of its encoded protein was further analyzed. Most of DLBCL (26/33, 78.8%) with bcl-6 gene rearrangement presented with overexpression of its encoded protein, which was higher than those without bcl-6 gene rearrangement (53/84, 62.4%, P=0.088). DLBCL with bcl-6 gene rearrangement (24/33, 72.7%) more frequently expressed cyclin D3, and had a higher proliferative activity than those without bcl-6 gene rearrangement (37/81, 45.7% , P=0.009). Twenty-nine of 33 (87.9%) cases of DLBCL with bcl-6 gene rearrangement presented with advanced stage (Ann Arbor stage III/IV), which was higher than those without bcl-6 gene rearrangement (65/85, 76.5% , P=0.167). Univariate Cox proportional hazards regression analysis showed that bcl-6 gene rearrangement was associated with an increased relative risk (at 1.842) of death in DLBCL cases compared with those without bcl-6 gene rearrangement. CONCLUSION: Overexpression of bcl-6 protein caused by bcl-6 gene rearrangement may play some important roles in the development and/or progression of a subset of DLBCL. FAU - Xu, Fang-ping AU - Xu FP AD - Department of Pathology and Laboratory Medicine, Guangdong Provincial People's Hospital, Guangzhou, 510080, China. FAU - Liu, Yan-hui AU - Liu YH FAU - Luo, Xin-lan AU - Luo XL FAU - Zhuang, Heng-guo AU - Zhuang HG FAU - Li, Li AU - Li L FAU - Luo, Dong-lan AU - Luo DL FAU - Xu, Jie AU - Xu J FAU - Zhang, Fen AU - Zhang F FAU - Zhang, Ming-hui AU - Zhang MH FAU - Du, Xin AU - Du X FAU - Li, Wen-yu AU - Li WY LA - chi PT - English Abstract PT - Journal Article PL - China TA - Zhonghua Bing Li Xue Za Zhi JT - Zhonghua bing li xue za zhi = Chinese journal of pathology JID - 0005331 RN - 0 (Cyclin D3) RN - 0 (Proto-Oncogene Proteins c-bcl-6) SB - IM MH - B-Lymphocytes/pathology MH - Chromosomes, Human, Pair 14 MH - Cyclin D3/*genetics MH - Gene Rearrangement MH - Humans MH - Immunohistochemistry MH - In Situ Hybridization, Fluorescence MH - Lymphoma, B-Cell/diagnosis/*genetics MH - Lymphoma, Large B-Cell, Diffuse/diagnosis/*genetics/metabolism/pathology MH - Neoplasm Staging MH - Prognosis MH - Proto-Oncogene Proteins c-bcl-6/*genetics MH - Translocation, Genetic EDAT- 2008/11/27 09:00 MHDA- 2010/09/30 06:00 CRDT- 2008/11/27 09:00 PHST- 2008/11/27 09:00 [pubmed] PHST- 2010/09/30 06:00 [medline] PHST- 2008/11/27 09:00 [entrez] PST - ppublish SO - Zhonghua Bing Li Xue Za Zhi. 2008 Jun;37(6):371-6.