PMID- 19031943
OWN - NLM
STAT- MEDLINE
DCOM- 20090128
LR  - 20081126
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 28
IP  - 5B
DP  - 2008 Sep-Oct
TI  - TUNEL as a tumor marker of tongue cancer.
PG  - 2981-6
AB  - Squamous cell carcinoma (SCC) of the tongue has an increasing incidence, a high 
      morbidity rate and a 50% 5-year mortality rate. We analyzed the rate of apoptosis 
      using TDT-mediated dUTP-biotin nick end-labeling (TUNEL), p53 and heparanase in 
      73 patients with tongue cancer by immonohistochemistry, and tested data for 
      correlation with survival, tumor size, grade and metastasis. TUNEL staining was 
      strong in 54% of the tumors; the remainder lacked staining, as did all healthy 
      control tissues. Significant correlations were found between TUNEL staining level 
      and p53 expression rates (p=0.016) and between TUNEL and heparanase (p=0.039). 
      Moreover, while the cumulative 5-year survival probability for tumors not stained 
      for TUNEL and p53 was 63%, but dropped to 34% with TUNEL staining; for lack of 
      TUNEL and heparanase (=0), 5-year survival rate was 50%, while staining presence 
      (>0) reduced survival to 34%. TUNEL joins other biomarkers in indicating 
      prognosis of understanding pathogenesis of tongue cancer.
FAU - Ben-Izhak, Ofer
AU  - Ben-Izhak O
AD  - Department of Pathology, Rambam Medical Center, Haifa, Israel.
FAU - Laster, Zvi
AU  - Laster Z
FAU - Akrish, Sharon
AU  - Akrish S
FAU - Cohen, Golan
AU  - Cohen G
FAU - Nagler, Rafael M
AU  - Nagler RM
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (TP53 protein, human)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 3.2.1.- (heparanase)
RN  - EC 3.2.1.31 (Glucuronidase)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Amino Acid Sequence
MH  - Apoptosis/genetics
MH  - Carcinoma, Squamous Cell/genetics/*metabolism/*pathology
MH  - Cohort Studies
MH  - Female
MH  - Glucuronidase/*biosynthesis/genetics
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Prognosis
MH  - Survival Rate
MH  - Tongue Neoplasms/genetics/*metabolism/*pathology
MH  - Tumor Suppressor Protein p53/*biosynthesis/genetics
MH  - Young Adult
EDAT- 2008/11/27 09:00
MHDA- 2009/01/29 09:00
CRDT- 2008/11/27 09:00
PHST- 2008/11/27 09:00 [pubmed]
PHST- 2009/01/29 09:00 [medline]
PHST- 2008/11/27 09:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 2008 Sep-Oct;28(5B):2981-6.