PMID- 19032361
OWN - NLM
STAT- MEDLINE
DCOM- 20090330
LR  - 20161125
IS  - 1365-2222 (Electronic)
IS  - 0954-7894 (Linking)
VI  - 39
IP  - 2
DP  - 2009 Feb
TI  - Anti-allergic effects of PG102, a water-soluble extract prepared from Actinidia 
      arguta, in a murine ovalbumin-induced asthma model.
PG  - 280-9
LID - 10.1111/j.1365-2222.2008.03124.x [doi]
AB  - BACKGROUND: Asthma is a chronic inflammatory disease of the lung and its 
      incidence has been increasing around the world. We previously reported that oral 
      administration of a water-soluble extract prepared from Actinidia arguta, 
      code-named PG102, could modulate the level of Th1 and Th2 cytokines and suppress 
      the production of immunoglobulin E (IgE) in the ovalbumin (OVA)-immunized murine 
      model as well as in the in vitro cell culture system, and furthermore could 
      significantly improve dermatitis conditions in the NC/Nga murine model. These 
      data suggested that PG102 might have therapeutic effects in a broad range of 
      allergic diseases. OBJECTIVE: To assess the possible anti-allergic effects of 
      PG102 in the OVA-induced murine asthma model. METHODS: The quality of PG102 was 
      standardized, using its effects on the production of IgE, IL-5, and IL-13, in in 
      vitro cell culture systems. To test effects on asthma, BALB/c mice were orally 
      administrated with PG102, followed by OVA sensitization and challenge to induce 
      asthmatic symptoms. Airway hyperresponsiveness (AHR), bronchoalveolar lavage 
      fluid, serum, and lung tissue were analysed by using various methods. RESULTS: 
      PG102 could decrease the level of IgE, IL-5, and IL-13 in in vitro cell culture 
      systems with IC(50) being 1.12-1.43 mg/mL. PG102 could ameliorate asthmatic 
      symptoms, including AHR and eosinophilia in the lungs. Such improvement of 
      asthmatic symptoms by PG102 was accompanied by the down-regulation of IL-5 and 
      IgE. In PG102-treated mice, high level expression of heme oxygenase-1, a potent 
      anti-inflammatory enzyme, was observed in alveolar inflammatory cells, while the 
      mRNA levels of foxp3, TGF-beta1, and IL-10, important markers for regulatory T 
      cells, were also up-regulated in the lung tissue. CONCLUSIONS: PG102 may have 
      potential as a safe and effective reagent for the prevention or treatment of 
      asthma.
FAU - Kim, D
AU  - Kim D
AD  - School of Biological Sciences, Seoul National University, Seoul, Korea.
FAU - Kim, S H
AU  - Kim SH
FAU - Park, E-J
AU  - Park EJ
FAU - Kang, C-Y
AU  - Kang CY
FAU - Cho, S-H
AU  - Cho SH
FAU - Kim, S
AU  - Kim S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081117
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Cytokines)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Foxp3 protein, mouse)
RN  - 0 (Plant Extracts)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 130068-27-8 (Interleukin-10)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
SB  - IM
MH  - Actinidia/*chemistry
MH  - Animals
MH  - Asthma/chemically induced/*drug therapy/immunology/physiopathology
MH  - B-Lymphocytes/drug effects
MH  - Bronchial Hyperreactivity/physiopathology
MH  - Bronchoalveolar Lavage Fluid/chemistry/cytology
MH  - Cytokines/analysis/metabolism
MH  - *Disease Models, Animal
MH  - Eosinophils/cytology
MH  - Female
MH  - Forkhead Transcription Factors/genetics
MH  - Fruit/chemistry
MH  - Gene Expression/drug effects/genetics
MH  - Heme Oxygenase-1/genetics/metabolism
MH  - Humans
MH  - Immunoglobulin E/blood/metabolism
MH  - Interleukin-10/genetics
MH  - Lung/drug effects/metabolism/pathology
MH  - Mast Cells/drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/immunology
MH  - Phytotherapy/*methods
MH  - Plant Extracts/pharmacology/*therapeutic use
MH  - Respiratory Function Tests
MH  - T-Lymphocytes/drug effects/metabolism
MH  - Transforming Growth Factor beta1/genetics
EDAT- 2008/11/27 09:00
MHDA- 2009/03/31 09:00
CRDT- 2008/11/27 09:00
PHST- 2008/11/27 09:00 [pubmed]
PHST- 2009/03/31 09:00 [medline]
PHST- 2008/11/27 09:00 [entrez]
AID - CEA3124 [pii]
AID - 10.1111/j.1365-2222.2008.03124.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2009 Feb;39(2):280-9. doi: 10.1111/j.1365-2222.2008.03124.x. 
      Epub 2008 Nov 17.