PMID- 19032374
OWN - NLM
STAT- MEDLINE
DCOM- 20100407
LR  - 20151119
IS  - 1751-553X (Electronic)
IS  - 1751-5521 (Linking)
VI  - 32
IP  - 1 Pt 1
DP  - 2010 Feb
TI  - Modified ELISPOT assay may predict T-cell hyporesponsiveness to non-inherited 
      maternal antigens.
PG  - e163-8
LID - 10.1111/j.1751-553X.2008.01121.x [doi]
AB  - Clinical reports have suggested the existence of immunological tolerance to 
      noninherited maternal antigens (NIMA) in human leukocyte antigen (HLA) mismatched 
      allogeneic stem cell transplantation (allo-SCT). We studied the T-cell reactivity 
      using IFN-gamma enzyme-linked immunospot (ELISPOT) assay in three HLA fully 
      matched allo-SCT cases and one healthy volunteer family case. In HLA fully 
      matched allo-SCT cases, ELISPOT assay could detect the hyporesponsiveness of T 
      cells from donors to the B cells from recipients. Moreover, ELISPOT assay showed 
      that the T cells from an individual responded to B cell from his mother 
      significantly weakly than those from an unrelated HLA-haploidentical individual. 
      These observations suggest that our IFN-gamma ELISPOT assay-based method may 
      predict the presence of immunological tolerance to NIMA.
FAU - Taniguchi, K
AU  - Taniguchi K
AD  - Division of Hematology and Oncology, Department of Medicine, Kyoto Prefectural 
      University of Medicine, Kyoto, Japan.
FAU - Shimazaki, C
AU  - Shimazaki C
FAU - Ochiai, N
AU  - Ochiai N
FAU - Maruya, E
AU  - Maruya E
FAU - Akatsuka, Y
AU  - Akatsuka Y
FAU - Ashihara, E
AU  - Ashihara E
FAU - Maekawa, T
AU  - Maekawa T
FAU - Taniwaki, M
AU  - Taniwaki M
FAU - Saji, H
AU  - Saji H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081121
PL  - England
TA  - Int J Lab Hematol
JT  - International journal of laboratory hematology
JID - 101300213
RN  - 0 (HLA Antigens)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - B-Lymphocytes/immunology
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Female
MH  - HLA Antigens/*immunology
MH  - Hematopoietic Stem Cell Transplantation/methods
MH  - Humans
MH  - Immune Tolerance/*immunology
MH  - Interferon-gamma
MH  - Male
MH  - Maternal-Fetal Exchange/immunology
MH  - Pregnancy
MH  - T-Lymphocytes/*immunology
MH  - Transplantation, Homologous/immunology
EDAT- 2008/11/27 09:00
MHDA- 2010/04/08 06:00
CRDT- 2008/11/27 09:00
PHST- 2008/11/27 09:00 [pubmed]
PHST- 2010/04/08 06:00 [medline]
PHST- 2008/11/27 09:00 [entrez]
AID - CLH1121 [pii]
AID - 10.1111/j.1751-553X.2008.01121.x [doi]
PST - ppublish
SO  - Int J Lab Hematol. 2010 Feb;32(1 Pt 1):e163-8. doi: 
      10.1111/j.1751-553X.2008.01121.x. Epub 2008 Nov 21.