PMID- 19032437
OWN - NLM
STAT- MEDLINE
DCOM- 20110804
LR  - 20181201
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 44
IP  - 5
DP  - 2009 Oct
TI  - Uterine expression of epidermal growth factor family during the course of 
      pregnancy in pigs.
PG  - 797-804
LID - 10.1111/j.1439-0531.2008.01082.x [doi]
AB  - To stably maintain pregnancy, several genes are expressed in the uterus. In 
      particular, the endometrial expression of genes encoding growth factors appears 
      to play a key role in maternal-foetal communication. The previous studies 
      characterized the endometrial expression kinetics of the genes encoding epidermal 
      growth factor (EGF), its receptor (EGFR), transforming growth factor-alpha 
      (TGF-alpha), amphiregulin (Areg), heparin-binding (Hb) EGF and calbindin-D9k 
      (CaBP-9k) in pigs during implantation. Here, we further characterized the 
      expression patterns of these molecules during the entire porcine pregnancy. 
      Porcine uteri were collected at pregnancy days (PD) 12, 15, 30, 60, 90 and 110 
      and subjected to RT-PCR. EGF and EGFR showed similar expression patterns, being 
      highly expressed around implantation and then disappearing. TGF-alpha and Areg 
      expression levels rose steadily until they peaked at PD30, after which they 
      gradually decreased to PD12 levels. This Areg mRNA expression pattern was 
      confirmed by real-time PCR and similar Areg protein expression patterns were 
      observed. Immunohistochemical analysis of PD60 uteri revealed Areg in the 
      glandular and luminal epithelial cells. Hb EGF was steadily expressed throughout 
      the entire pregnancy, while CaBP-9k was expressed strongly on PD12, and then 
      declined sharply on PD15 before recovering slightly for the remainder of the 
      pregnancy. Thus, the EGF family may play a key role during implantation in pigs. 
      In addition, CaBP-9k may help to maintain uterine quiescence during pregnancy by 
      sequestering cytoplasmic Ca(2+).
FAU - Kim, Y-J
AU  - Kim YJ
AD  - Laboratory of Veterinary Biochemistry and Molecular Biology, Chungbuk National 
      University, Cheongju, Chungbuk, Republic of Korea.
FAU - Lee, G-S
AU  - Lee GS
FAU - Hyun, S-H
AU  - Hyun SH
FAU - Ka, H-H
AU  - Ka HH
FAU - Choi, K-C
AU  - Choi KC
FAU - Lee, C-K
AU  - Lee CK
FAU - Jeung, E-B
AU  - Jeung EB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081113
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
RN  - 0 (Amphiregulin)
RN  - 0 (Calbindins)
RN  - 0 (Glycoproteins)
RN  - 0 (Heparin-binding EGF-like Growth Factor)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (S100 Calcium Binding Protein G)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Amphiregulin
MH  - Animals
MH  - Calbindins
MH  - Embryo Implantation/physiology
MH  - Endometrium/*chemistry
MH  - Epidermal Growth Factor/*genetics
MH  - ErbB Receptors/genetics
MH  - Female
MH  - *Gene Expression
MH  - Gestational Age
MH  - Glycoproteins/analysis/genetics
MH  - Heparin-binding EGF-like Growth Factor
MH  - Intercellular Signaling Peptides and Proteins/analysis/genetics
MH  - Polymerase Chain Reaction/veterinary
MH  - Pregnancy
MH  - RNA, Messenger/analysis
MH  - S100 Calcium Binding Protein G/genetics
MH  - *Swine
MH  - Transforming Growth Factor alpha/genetics
EDAT- 2008/11/27 09:00
MHDA- 2011/08/05 06:00
CRDT- 2008/11/27 09:00
PHST- 2008/11/27 09:00 [pubmed]
PHST- 2011/08/05 06:00 [medline]
PHST- 2008/11/27 09:00 [entrez]
AID - RDA1082 [pii]
AID - 10.1111/j.1439-0531.2008.01082.x [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2009 Oct;44(5):797-804. doi: 
      10.1111/j.1439-0531.2008.01082.x. Epub 2008 Nov 13.