PMID- 19032979
OWN - NLM
STAT- MEDLINE
DCOM- 20090415
LR  - 20211020
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 158
IP  - 2
DP  - 2009 Jan 23
TI  - Monocyte chemoattractant protein-1 in the choroid plexus: a potential link 
      between vascular pro-inflammatory mediators and the CNS during peripheral tissue 
      inflammation.
PG  - 885-95
LID - 10.1016/j.neuroscience.2008.10.047 [doi]
AB  - During peripheral tissue inflammation, inflammatory processes in the CNS can be 
      initiated by blood-borne pro-inflammatory mediators. The choroid plexus, the site 
      of cerebrospinal fluid (CSF) production, is a highly specialized interface 
      between the vascular system and CNS, and thus, this structure may be an important 
      element in communication between the vascular compartment and the CNS during 
      peripheral tissue inflammation. We investigated the potential participation of 
      the choroid plexus in this process during peripheral tissue inflammation by 
      examining expression of the small inducible cytokine A2 (SCYA2) gene which codes 
      for monocyte chemoattractant protein-1 (MCP-1). MCP-1 protein was previously 
      reported to be induced in a variety of cells during peripheral tissue 
      inflammation. In the basal state, SCYA2 is highly expressed in the choroid plexus 
      as compared with other rat CNS tissues. During hind paw inflammation, SCYA2 
      expression was significantly elevated in choroid plexus, whereas it remained 
      unchanged in a variety of brain regions. The SCYA2-expressing cells were strongly 
      associated with the choroid plexus as vascular depletion of blood cells by 
      whole-body saline flush did not significantly alter SCYA2 expression in the 
      choroid plexus. In situ hybridization suggested that the SCYA2-expressing cells 
      were localized to the choroid plexus stroma. To elucidate potential molecular 
      mechanisms of SCYA2 increase, we examined genes in the nuclear factor-kappa B 
      (NF-kappaB) signaling cascade including tumor necrosis factor-alpha (TNF-alpha), 
      interleukin-1beta (IL-1beta) and inhibitor of kappa B alpha (IkappaBalpha) in 
      choroid tissue. Given that we also detected increased levels of MCP-1 protein by 
      ELISA, we sought to identify potential downstream targets of MCP-1 and observed 
      altered expression levels of mRNAs encoding tight junction proteins TJP2 and 
      claudin 5. Finally, we detected a substantial up-regulation of the transcript 
      encoding endothelial leukocyte adhesion molecule 1 (E-selectin), a molecule which 
      could participate in leukocyte recruitment to the choroid plexus along with 
      MCP-1. Together, these results suggest that profound changes occur in the choroid 
      plexus during peripheral tissue inflammation, likely initiated by blood-borne 
      inflammatory mediators, which may modify events in CNS.
FAU - Mitchell, K
AU  - Mitchell K
AD  - Neurobiology and Pain Therapeutics Section, National Institute of Dental and 
      Craniofacial Research, National Institutes of Health, Department of Health and 
      Human Services, Building 49, Room 1C20, 49 Convent Drive, MSC 4410, Bethesda, MD 
      20892-4410, USA.
FAU - Yang, H-Y T
AU  - Yang HY
FAU - Berk, J D
AU  - Berk JD
FAU - Tran, J H
AU  - Tran JH
FAU - Iadarola, M J
AU  - Iadarola MJ
LA  - eng
GR  - Z01 DE000688-11/ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20081107
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 9000-07-1 (Carrageenan)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Brain/metabolism/*pathology
MH  - Carrageenan
MH  - Caspase 1/genetics/metabolism
MH  - Cell Adhesion Molecules/genetics/metabolism
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Choroid Plexus/*metabolism
MH  - Cytokines/genetics/*metabolism
MH  - Disease Models, Animal
MH  - Gene Expression Regulation/drug effects/*physiology
MH  - Inflammation/chemically induced/*pathology
MH  - Male
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
PMC - PMC2668531
MID - NIHMS94269
EDAT- 2008/11/27 09:00
MHDA- 2009/04/16 09:00
PMCR- 2010/01/23
CRDT- 2008/11/27 09:00
PHST- 2008/07/07 00:00 [received]
PHST- 2008/10/17 00:00 [revised]
PHST- 2008/10/28 00:00 [accepted]
PHST- 2008/11/27 09:00 [entrez]
PHST- 2008/11/27 09:00 [pubmed]
PHST- 2009/04/16 09:00 [medline]
PHST- 2010/01/23 00:00 [pmc-release]
AID - S0306-4522(08)01590-X [pii]
AID - 10.1016/j.neuroscience.2008.10.047 [doi]
PST - ppublish
SO  - Neuroscience. 2009 Jan 23;158(2):885-95. doi: 10.1016/j.neuroscience.2008.10.047. 
      Epub 2008 Nov 7.