PMID- 19033711
OWN - NLM
STAT- MEDLINE
DCOM- 20090511
LR  - 20090417
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Linking)
VI  - 149
IP  - 1
DP  - 2009
TI  - Contrasting immunological effects of two disparate dusts - preliminary 
      observations.
PG  - 81-90
LID - 10.1159/000176310 [doi]
AB  - BACKGROUND: Modern lifestyle and urbanization have been associated with a raised 
      risk for atopic diseases whereas early and long-term exposure to a farm 
      environment confers protection against atopic sensitization. Immunomodulatory 
      potential and microbiological characteristics of settled airborne dust from an 
      urban house and a barn were examined. METHODS: Pulmonary inflammation was induced 
      in mice by repeated intranasal administration of dusts. Monocyte-derived human 
      dendritic cells (moDCs) were exposed to dusts followed by coculture with purified 
      naive T cells. Cytokine/chemokine mRNA and protein levels were analyzed by 
      real-time polymerase chain reaction, enzyme-linked immunosorbent assay and flow 
      cytometry. The dusts were analyzed by cloning and sequencing of 16S rRNA genes 
      (290 sequences) for DNA, lipids, endotoxin and beta-glucan, by live-dead 
      staining, viable counting, isolation and identification of pure cultures (n = 
      76). RESULTS: Repeated exposure to house dust elicited pulmonary eosinophilia in 
      mice whereas exposure to barn dust elicited neutrophilic and lymphocytic airway 
      inflammation. Stimulation of moDCs with urban house dust elicited expression of 
      Th2-promoting OX40L and Jagged-1 costimulatory molecules. Dendritic cells (DCs) 
      exposed to house dust directed naive T cells towards Th2 responses. Exposure of 
      DCs to barn dust elicited the development of Th1-dominated immune responses. 
      Urban house dust contained bacterial debris almost exclusively of human commensal 
      species (corynebacteria, streptococci) whereas barn dust comprised mainly intact, 
      viable bacteria of high diversity and no commensal species. CONCLUSION: Contact 
      to debris originating from human commensal bacteria in urban house dust elicited 
      a Th2-type response whereas barn dust with high bacterial diversity directed the 
      cells towards a Th1 response.
CI  - 2008 S. Karger AG, Basel.
FAU - Alenius, Harri
AU  - Alenius H
AD  - Finnish Institute of Occupational Health, Helsinki, Finland. Harri.Alenius@ttl.fi
FAU - Pakarinen, Jaakko
AU  - Pakarinen J
FAU - Saris, Ossian
AU  - Saris O
FAU - Andersson, Maria A
AU  - Andersson MA
FAU - Leino, Marina
AU  - Leino M
FAU - Sirola, Kristiina
AU  - Sirola K
FAU - Majuri, Marja-Leena
AU  - Majuri ML
FAU - Niemela, Jari
AU  - Niemela J
FAU - Matikainen, Sampsa
AU  - Matikainen S
FAU - Wolff, Henrik
AU  - Wolff H
FAU - von Hertzen, Leena
AU  - von Hertzen L
FAU - Makela, Mika
AU  - Makela M
FAU - Haahtela, Tari
AU  - Haahtela T
FAU - Salkinoja-Salonen, Mirja
AU  - Salkinoja-Salonen M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081126
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Dust)
SB  - IM
MH  - Air Pollution, Indoor/*adverse effects/analysis
MH  - Animals
MH  - Bacteria/immunology/isolation & purification
MH  - Dendritic Cells/immunology/microbiology
MH  - Dust/analysis/*immunology
MH  - Female
MH  - Humans
MH  - Inhalation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Phylogeny
MH  - Pneumonia/*immunology/microbiology
MH  - Pulmonary Eosinophilia/immunology/microbiology
MH  - T-Lymphocytes/immunology/microbiology
MH  - Th1 Cells/*immunology/microbiology
MH  - Th2 Cells/*immunology/microbiology
EDAT- 2008/11/27 09:00
MHDA- 2009/05/12 09:00
CRDT- 2008/11/27 09:00
PHST- 2008/05/14 00:00 [received]
PHST- 2008/07/23 00:00 [accepted]
PHST- 2008/11/27 09:00 [pubmed]
PHST- 2009/05/12 09:00 [medline]
PHST- 2008/11/27 09:00 [entrez]
AID - 000176310 [pii]
AID - 10.1159/000176310 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2009;149(1):81-90. doi: 10.1159/000176310. Epub 2008 
      Nov 26.