PMID- 19034982
OWN - NLM
STAT- MEDLINE
DCOM- 20090519
LR  - 20211020
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 14
IP  - 43
DP  - 2008 Nov 21
TI  - Non-thermal ablation of rabbit liver VX2 tumor by pulsed high intensity focused 
      ultrasound with ultrasound contrast agent: Pathological characteristics.
PG  - 6743-7
AB  - AIM: To investigate the pathological characteristics of non-thermal damage 
      induced by pulsed high intensity focused ultrasound (PHIFU) combined with 
      ultrasound contrast agent (UCA), SonoVue (Bracco SpA, Milan, Italy) in rabbit 
      liver VX2 tumor. METHODS: Liver VX2 tumor models were established in 20 rabbits, 
      which were divided randomly into PHIFU combined with ultrasound contrast agent 
      group (PHIFU + UCA group) and sham group. In the PHIFU + UCA group, 0.2 mL of 
      SonoVue was injected intravenously into the tumor, followed by ultrasound 
      exposure of I(SP) 5900 W/cm(2). The rabbits were sacrificed one day after 
      ultrasound exposure. Specimens of the exposed tumor tissues were obtained and 
      observed pathologically under light microscope and transmission electron 
      microscope. The remaining tumor tissues were sent for 2,3,5-Triphenyltetrazolium 
      chloride (TTC) staining. RESULTS: Before TTC staining, tumor tissues in both the 
      sham and the PHIFU + UCA groups resembled gray fish meat. After TTC staining, the 
      tumor tissues were uniformly stained red, with a clear boundary between tumor 
      tissue and normal tissue. Histological examination showed signs of tumor cell 
      injury in PHIFU + UCA group, with cytoplasmic vacuoles of various sizes, 
      chromatin margination and karyopyknosis. Electron microscopic examination 
      revealed tumor cell volume reduction, karyopyknosis, chromatin margination, 
      intercellular space widening, the presence of high electron-density apoptotic 
      bodies and vacuoles in cytoplasm. CONCLUSION: The non-thermal effects of PHIFU 
      combined with UCA can be used to ablate rabbit liver VX2 tumors.
FAU - Zhou, Cheng-Wen
AU  - Zhou CW
AD  - Bioengineering College, Chongqing University, 174 Shazhengjie St, Shapingba 
      District, Chongqing 400044, China. lifaqi70@163.com
FAU - Li, Fa-Qi
AU  - Li FQ
FAU - Qin, Yan
AU  - Qin Y
FAU - Liu, Chun-Mei
AU  - Liu CM
FAU - Zheng, Xiao-Lin
AU  - Zheng XL
FAU - Wang, Zhi-Biao
AU  - Wang ZB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Chromatin)
RN  - 0 (Contrast Media)
SB  - IM
MH  - Ablation Techniques/*methods
MH  - Animals
MH  - Carcinoma, Squamous Cell/*pathology/*surgery/ultrastructure
MH  - Chromatin/ultrastructure
MH  - *Contrast Media
MH  - Female
MH  - Liver Neoplasms/*pathology/*surgery/ultrastructure
MH  - Male
MH  - Rabbits
MH  - Ultrasonic Therapy/*methods
MH  - Vacuoles/ultrastructure
PMC - PMC2773321
EDAT- 2008/11/27 09:00
MHDA- 2009/05/20 09:00
PMCR- 2008/11/21
CRDT- 2008/11/27 09:00
PHST- 2008/11/27 09:00 [pubmed]
PHST- 2009/05/20 09:00 [medline]
PHST- 2008/11/27 09:00 [entrez]
PHST- 2008/11/21 00:00 [pmc-release]
AID - 10.3748/wjg.14.6743 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2008 Nov 21;14(43):6743-7. doi: 10.3748/wjg.14.6743.