PMID- 19035265
OWN - NLM
STAT- MEDLINE
DCOM- 20110519
LR  - 20081127
IS  - 1673-0860 (Print)
IS  - 1673-0860 (Linking)
VI  - 43
IP  - 9
DP  - 2008 Sep
TI  - [Global gene expression profiling of laryngeal squamous cell carcinoma by laser 
      capture microdissection and complementary DNA microarrays].
PG  - 696-700
AB  - OBJECTIVE: To examine the gene expression profile of laryngeal squamous cell 
      carcinoma (LSCC) by combination of laser capture microdissection (LCM) and 
      microarray and to identify genetic changes in disease pathogenesis. METHODS: The 
      study analysed 8 matched pairs of specimens of glottic carcinoma of larynx and 
      histologically normal epithelium tissues adjacent to the carcinoma preserved in 
      the RNA later reagent. A genome-wide transcriptome analysis was performed by 
      probing 16 cDNA microarrays with fluorescent-labeled amplified RNA derived from 
      laser capture microdissected cells. Real-time quantitative (RT-PCR) of tissue 
      microarray was used to validate the reliability of cDNA microarrays. RESULTS: 
      Significant analysis of microarray (SAM) software and hierarchical cluster 
      analysis of the expressed genes showed that 2351 genes was significantly 
      expressed respectively according to different analysis method (false discover 
      rate = 0.63%). A selected set of MMP12, KRT16, RARB, PRB1 genes was identified to 
      be consistent with array data by RT-PCR. CONCLUSIONS: The analysis of gene 
      ontology and pathway distributions futher highlighted genes that may be 
      critically important to laryngeal carcinogenesis. The results strongly suggest 
      that this new approach may facilitate the identification of clinical molecular 
      markers of disease and novel potential therapeutic targets for LSCC.
FAU - Ma, Li-Juan
AU  - Ma LJ
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital, 
      Central South University, Changsha 410008, China.
FAU - Tian, Yong-Quan
AU  - Tian YQ
FAU - Xiao, Jian-Yun
AU  - Xiao JY
FAU - Li, Wei
AU  - Li W
FAU - Zhang, Hua
AU  - Zhang H
FAU - Zhang, Xin
AU  - Zhang X
FAU - Huang, Dong-Hai
AU  - Huang DH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
JT  - Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of 
      otorhinolaryngology head and neck surgery
JID - 101247574
SB  - IM
MH  - Aged
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Gene Expression Profiling/*methods
MH  - Gene Expression Regulation, Neoplastic
MH  - Genome
MH  - Glottis
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - Lasers
MH  - Male
MH  - Microdissection/methods
MH  - Middle Aged
MH  - Oligonucleotide Array Sequence Analysis/methods
EDAT- 2008/11/28 09:00
MHDA- 2011/05/20 06:00
CRDT- 2008/11/28 09:00
PHST- 2008/11/28 09:00 [pubmed]
PHST- 2011/05/20 06:00 [medline]
PHST- 2008/11/28 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2008 Sep;43(9):696-700.