PMID- 19035322
OWN - NLM
STAT- MEDLINE
DCOM- 20090106
LR  - 20161021
IS  - 1000-8721 (Print)
IS  - 1000-8721 (Linking)
VI  - 24
IP  - 5
DP  - 2008 Sep
TI  - [Pseudotyping of murine leukemia virus particles with porcine reproductive and 
      respiratory syndrome virus M protein-mediated E protein].
PG  - 345-51
AB  - For constructing pcDNA-ORF5, pcDNA-ORF5-ORF6, pcDNA-ORF5/6, the ORF5 and ORF6 of 
      porcine reproductive and respiratory syndrome virus (PRRSV) were amplified by 
      RT-PCR, and transiently transfected into 293T cells by calciumphosphate 
      co-precipitation. After 48 h, 293T cells were collected and surveyed by flow 
      cytometry examination. The result indicated that the expression level of the E 
      protein that mediated by the M protein was higher than that of the E protein 
      expressed independently. Then pcDNA-ORF5, pcDNA-ORF5-ORF6, pcDNA-ORF5/6 were 
      respectivly co-transfected into 293T cells with pHIT60 (include MuLV structural 
      genes,namely gag and pol) and pHIT111 (retroviral genome, containing LacZ as a 
      reporter). The supernatants were harvested 48 h post-transfection,and the 
      analysis of the characteristic of the pseudotyping virions was performed by 
      Western blot and infection test. The result indicated that the E proteins were 
      expressed on the virions, and incorporated into the retroviral virions. Infection 
      test were performed on Marc-145 and PAM, all the cells infected were Lac Z 
      positive. These results indicated the pseudotype virions of MuLV-E and MuLV-E/M 
      were infectious, and higher infectivity was achieved by MuLV-E/M.
FAU - Xia, Ping-An
AU  - Xia PA
AD  - The College of Animal Husbandy and Veterinary, Henan Agricultural University, 
      Zhengzhou 450002, China. xpa-88@sohu.com
FAU - Dang, Zhan-Guo
AU  - Dang ZG
FAU - Zhou, Bin
AU  - Zhou B
FAU - Chen, Pu-Yanz
AU  - Chen PY
FAU - Cui, Bao-An
AU  - Cui BA
FAU - Qiu, Huang
AU  - Qiu H
FAU - Lu, Gao-Feng
AU  - Lu GF
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Bing Du Xue Bao
JT  - Bing du xue bao = Chinese journal of virology
JID - 8803009
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Matrix Proteins)
SB  - IM
MH  - Flow Cytometry
MH  - Leukemia Virus, Murine/*genetics
MH  - Plasmids
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - Viral Envelope Proteins/genetics/*physiology
MH  - Viral Matrix Proteins/genetics/*physiology
MH  - Virion/*genetics
EDAT- 2008/11/28 09:00
MHDA- 2009/01/07 09:00
CRDT- 2008/11/28 09:00
PHST- 2008/11/28 09:00 [pubmed]
PHST- 2009/01/07 09:00 [medline]
PHST- 2008/11/28 09:00 [entrez]
PST - ppublish
SO  - Bing Du Xue Bao. 2008 Sep;24(5):345-51.