PMID- 19036073
OWN - NLM
STAT- MEDLINE
DCOM- 20090429
LR  - 20230829
IS  - 1538-7836 (Electronic)
IS  - 1538-7836 (Linking)
VI  - 7
IP  - 3
DP  - 2009 Mar
TI  - Short-term myocardial ischemia induces cardiac modified C-reactive protein 
      expression and proinflammatory gene (cyclo-oxygenase-2, monocyte chemoattractant 
      protein-1, and tissue factor) upregulation in peripheral blood mononuclear cells.
PG  - 485-93
LID - 10.1111/j.1538-7836.2008.03244.x [doi]
AB  - BACKGROUND: Prompt coronary thrombus resolution, reducing time of ischemia, 
      improves cardiac recovery. The factors triggered by ischemia that contribute to 
      the clinical outcome are not fully known. We hypothesize that unabated 
      inflammation due to cardiac ischemia may be a contributing factor. AIMS: As a 
      proof-of-concept, we evaluated the effect of short-term myocardial ischemia on 
      the local and systemic inflammatory response. METHODS: Pigs underwent either 
      90-min mid-left anterior descending (LAD) coronary artery balloon occlusion 
      (infarct size 25% +/- 1% left ventricle; 29% heart function deterioration) or a 
      sham-operation procedure. Peri-infarcted and non-ischemic cardiac tissue was 
      obtained for histopathologic, molecular and immunohistochemical analysis of 
      inflammatory markers [interleukin-6 (IL-6), tumor necrosis factor-alpha 
      (TNF-alpha), modified C-reactive protein (mCRP), and human alveolar macrophage-56 
      (HAM-56)]. Blood (femoral vein) was withdrawn prior to myocardial infarction (MI) 
      induction (t = 0) and at 30 and 90 min to evaluate: (i) systemic cytokine levels 
      (IL-6, TNF-alpha, CRP); (ii) proinflammatory gene and protein expression in 
      peripheral blood mononuclear cells (PBMCs) of tissue factor (TF), 
      cyclo-oxygenase-2 (Cox-2), monocyte chemoattractant protein-1 (MCP-1), and CRP; 
      and (iii) platelet activation (assessed by perfusion studies and RhoA 
      activation). RESULTS: Short-term ischemia triggered cardiac IL-6 and TNF-alpha 
      expression, recruitment of inflammatory cells, and mCRP expression in infiltrated 
      macrophages (P < 0.05 vs. t = 0 and sham). PBMC mRNA and protein expression of 
      MCP-1, Cox-2 and TF was significantly increased by ischemia, whereas no 
      differences were detected in CRP. Ischemia increased cardiac troponin-I, IL-6 and 
      TNF-alpha systemic levels, and was associated with higher platelet deposition and 
      RhoA activation (P < 0.001 vs. t = 0 and sham). CONCLUSION: Short-term myocardial 
      ischemia, even without atherosclerosis, induces an inflammatory phenotype by 
      inducing local recruitment of macrophages and systemic activation of mononuclear 
      cells, and renders platelets more susceptible to activation.
FAU - Vilahur, G
AU  - Vilahur G
AD  - Cardiovascular Research Centre, CSIC-ICCC, Hospital de la Santa Creu i Sant Pau, 
      Barcelona, Spain.
FAU - Hernandez-Vera, R
AU  - Hernandez-Vera R
FAU - Molins, B
AU  - Molins B
FAU - Casani, L
AU  - Casani L
FAU - Duran, X
AU  - Duran X
FAU - Padro, T
AU  - Padro T
FAU - Badimon, L
AU  - Badimon L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Thromb Haemost
JT  - Journal of thrombosis and haemostasis : JTH
JID - 101170508
RN  - 0 (Biomarkers)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Immunologic Factors)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - 9035-58-9 (Thromboplastin)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - C-Reactive Protein/*genetics/metabolism
MH  - Chemokine CCL2/genetics/metabolism
MH  - Cyclooxygenase 2/genetics/metabolism
MH  - Cytokines
MH  - Immune System/cytology
MH  - Immunologic Factors/*genetics/metabolism
MH  - Inflammation
MH  - Myocardial Ischemia/immunology/*metabolism/pathology
MH  - Platelet Activation
MH  - Swine
MH  - Thromboplastin/genetics/metabolism
MH  - Up-Regulation/*genetics
EDAT- 2008/11/28 09:00
MHDA- 2009/04/30 09:00
CRDT- 2008/11/28 09:00
PHST- 2008/11/28 09:00 [pubmed]
PHST- 2009/04/30 09:00 [medline]
PHST- 2008/11/28 09:00 [entrez]
AID - S1538-7836(22)12267-1 [pii]
AID - 10.1111/j.1538-7836.2008.03244.x [doi]
PST - ppublish
SO  - J Thromb Haemost. 2009 Mar;7(3):485-93. doi: 10.1111/j.1538-7836.2008.03244.x.