PMID- 19036596
OWN - NLM
STAT- MEDLINE
DCOM- 20090428
LR  - 20200930
IS  - 0981-9428 (Print)
IS  - 0981-9428 (Linking)
VI  - 47
IP  - 2
DP  - 2009 Feb
TI  - Nitrogen and carbon relationships between the parasitic weed Orobanche foetida 
      and susceptible and tolerant faba bean lines.
PG  - 153-9
LID - 10.1016/j.plaphy.2008.10.004 [doi]
AB  - The parasitic weed Orobanche foetida (Poiret) is an emergent agronomical problem 
      on faba bean in Tunisia. The Tunisian breeding programs for faba bean resistance 
      to O. foetida have produced several tolerant lines including the line 
      XBJ90.03-16-1-1-1, which limits both parasite attachments to the host roots and 
      growth of the attached parasites. The present study aims to provide a better 
      understanding of the nutritional relationships between the parasite and this 
      tolerant line in comparison with the susceptible Bachaar genotype. Phloem saps of 
      faba bean were harvested using phloem exudation experiments. The major organic 
      compounds potentially transferred from both faba bean genotypes to the parasite 
      were identified as sucrose, raffinose, stachyose, citrate, malate, asparagine 
      (ASN), aspartate (ASP), glutamine, glutamate, serine, alanine and GABA. However, 
      the phloem exudates of the tolerant line were highly deficient in nitrogen when 
      compared to that of the susceptible line. When attached to roots of the tolerant 
      line, the parasite displayed limited activities of soluble invertases in 
      tubercles, and especially in shoots, suggesting that the low performance of the 
      broomrapes attached to the tolerant line resulted from a reduced capacity to 
      utilize the host-derived carbohydrates. On the other hand, the mechanisms 
      involved in the osmotic adjustment and primary metabolism of the parasite did not 
      differ significantly according to the host genotype: mineral cations, especially 
      potassium and calcium, predominated as the major osmotically-active compounds in 
      both tubercles and shoots; shoots accumulated preferentially hexoses as organic 
      solutes although tubercles accumulated preferentially starch and soluble amino 
      acids, especially ASP and ASN. This suggests an important role for a 
      glutamine-dependent asparagine synthetase (EC 6.3.5.4) in the N metabolism of the 
      parasite.
FAU - Abbes, Zouhaier
AU  - Abbes Z
AD  - Institut National de la Recherche Agronomique de Tunisie (INRAT), Laboratoire des 
      Grandes Cultures, Rue Hedi Karray, 2080 Ariana, Tunisia. 
      zouhaier.abbes@fst.rnu.tn
FAU - Kharrat, Mohamed
AU  - Kharrat M
FAU - Delavault, Philippe
AU  - Delavault P
FAU - Chaibi, Wided
AU  - Chaibi W
FAU - Simier, Philippe
AU  - Simier P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081101
PL  - France
TA  - Plant Physiol Biochem
JT  - Plant physiology and biochemistry : PPB
JID - 9882449
RN  - 0 (Amino Acids)
RN  - 0 (Minerals)
RN  - 0 (Plant Proteins)
RN  - 9005-25-8 (Starch)
RN  - EC 3.2.1.26 (beta-Fructofuranosidase)
RN  - EC 6.3.1.1 (Aspartate-Ammonia Ligase)
SB  - IM
CIN - Plant Signal Behav. 2009 Apr;4(4):336-8. PMID: 19794856
MH  - Amino Acids/metabolism
MH  - Aspartate-Ammonia Ligase/*metabolism
MH  - *Carbohydrate Metabolism
MH  - Genotype
MH  - Minerals/analysis
MH  - Orobanche/*metabolism
MH  - Osmolar Concentration
MH  - Phloem/*chemistry
MH  - Plant Proteins/*metabolism
MH  - Starch/analysis
MH  - Vicia faba/genetics/*metabolism
MH  - beta-Fructofuranosidase/metabolism
EDAT- 2008/11/28 09:00
MHDA- 2009/04/29 09:00
CRDT- 2008/11/28 09:00
PHST- 2008/05/05 00:00 [received]
PHST- 2008/10/15 00:00 [revised]
PHST- 2008/10/18 00:00 [accepted]
PHST- 2008/11/28 09:00 [pubmed]
PHST- 2009/04/29 09:00 [medline]
PHST- 2008/11/28 09:00 [entrez]
AID - S0981-9428(08)00195-2 [pii]
AID - 10.1016/j.plaphy.2008.10.004 [doi]
PST - ppublish
SO  - Plant Physiol Biochem. 2009 Feb;47(2):153-9. doi: 10.1016/j.plaphy.2008.10.004. 
      Epub 2008 Nov 1.