PMID- 1903696
OWN - NLM
STAT- MEDLINE
DCOM- 19910702
LR  - 20161123
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 128
IP  - 6
DP  - 1991 Jun
TI  - Tumor necrosis factor-alpha increases release of arachidonate and prolactin from 
      rat anterior pituitary cells.
PG  - 2791-8
AB  - We investigated the effect of tumor necrosis factor-alpha (TNF alpha), a product 
      of activated macrophages, on the release of arachidonate from dispersed anterior 
      pituitary cells. Primary cultures of anterior pituitary cells from rats were 
      preincubated with [3H]arachidonate to label their phospholipid-containing 
      components. The cells were then washed and incubated with vehicle or test agents, 
      and PRL release into the medium and [3H]arachidonate cleaved from phospholipid 
      were measured. TNF alpha significantly increased the release of both PRL and [3H] 
      arachidonate release in a time- and dose-dependent manner. Other cytokines, such 
      as interleukin-1 alpha, interleukin-1 beta, and gamma-interferon, had no effect 
      on [3H]arachidonate release. To define the role of calcium in TNF alpha-induced 
      arachidonate release, dispersed pituitary cells were incubated with low calcium 
      medium, which decreased arachidonate release in response to TNF alpha. TNF alpha 
      potentiated the release of [3H]arachidonate and PRL promoted by phospholipase-A2 
      and melittin, and markedly shifted the dose-response curve to the left. 
      Inhibitors of phospholipase-A2, such as p-bromophenacyl bromide and quinacrine, 
      had no effect on TNF alpha-induced [3H]arachidonate and PRL release. BW755C, an 
      inhibitor of the conversion of arachidonate to its metabolites, decreased TNF 
      alpha-induced PRL release, while indomethacin, a prostaglandin synthesis 
      inhibitor, had no effect on TNF alpha-induced PRL release. These data indicate 
      that arachidonate metabolites may be involved in the process of TNF alpha-induced 
      PRL release.
FAU - Koike, K
AU  - Koike K
AD  - Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
FAU - Hirota, K
AU  - Hirota K
FAU - Ohmichi, M
AU  - Ohmichi M
FAU - Kadowaki, K
AU  - Kadowaki K
FAU - Ikegami, H
AU  - Ikegami H
FAU - Yamaguchi, M
AU  - Yamaguchi M
FAU - Miyake, A
AU  - Miyake A
FAU - Tanizawa, O
AU  - Tanizawa O
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Arachidonic Acids)
RN  - 0 (Culture Media)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 20449-79-0 (Melitten)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 66000-40-6 (4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine)
RN  - 9002-62-4 (Prolactin)
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - EC 3.1.1.4 (Phospholipases A2)
RN  - SY7Q814VUP (Calcium)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - 4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine/pharmacology
MH  - Animals
MH  - Arachidonic Acid
MH  - Arachidonic Acids/*metabolism
MH  - Calcium/metabolism
MH  - Culture Media
MH  - Female
MH  - Indomethacin/pharmacology
MH  - Melitten/pharmacology
MH  - Phospholipases A/pharmacology
MH  - Phospholipases A2
MH  - Pituitary Gland, Anterior/cytology/*metabolism
MH  - Prolactin/*metabolism
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1991/06/01 00:00
MHDA- 1991/06/01 00:01
CRDT- 1991/06/01 00:00
PHST- 1991/06/01 00:00 [pubmed]
PHST- 1991/06/01 00:01 [medline]
PHST- 1991/06/01 00:00 [entrez]
AID - 10.1210/endo-128-6-2791 [doi]
PST - ppublish
SO  - Endocrinology. 1991 Jun;128(6):2791-8. doi: 10.1210/endo-128-6-2791.