PMID- 19037998
OWN - NLM
STAT- MEDLINE
DCOM- 20090123
LR  - 20111117
IS  - 1349-7006 (Electronic)
IS  - 1347-9032 (Linking)
VI  - 100
IP  - 1
DP  - 2009 Jan
TI  - Analysis of CHOP rearrangement in pleomorphic liposarcomas using fluorescence in 
      situ hybridization.
PG  - 82-7
LID - 10.1111/j.1349-7006.2008.01008.x [doi]
AB  - Pleomorphic liposarcoma (PLS) is an aggressive subtype of liposarcoma composed of 
      high-grade sarcoma with pleomorphic lipoblasts. PLS usually exhibits a 
      heterogeneous histology and sometimes has a myxoid or round cell area similar to 
      myxoid/round cell liposarcomas (MLS/RCs). Using fluorescence in situ 
      hybridization (FISH) analysis, we investigated the existence of CHOP split 
      signals in various histological areas of PLS including the MLS/RC-like feature 
      and also estimated the distribution of various signals with polyploidy and 
      amplification. Moreover, to detect CHOP fusion transcripts we performed nested 
      reverse transcription-polymerase chain reaction (RT-PCR). Seven PLSs and three 
      MLS/RCs were selected for FISH analysis using the locus-specific indicator CHOP 
      (12q13) dual color, break apart probe (Vysis, USA). The FISH analysis was applied 
      to formalin-fixed, paraffin-embedded tissue sections of representative areas in 
      all cases. Six of seven PLS cases showed the CHOP split signal ranging from 0.5% 
      to 3% of counted nuclei, while all cases of MLS/RC exhibited CHOP rearrangement 
      in more than 50% of counted nuclei. All cases of PLS showed a varied distribution 
      of extra signals with polyploidy and amplification in each histological area. No 
      CHOP fusion transcript was found in any case of PLS by nested RT-PCR. A CHOP 
      rearrangement in PLS should be recognized only as a representative part of 
      complex karyotypes, because the number of cells with split signals was minute 
      compared with that of MLS/RC, and the signals were found in any area despite 
      their histological differences. The cytogenetic background of PLS and that of 
      MLS/RC are obviously different despite histological similarity.
FAU - Sugita, Shintaro
AU  - Sugita S
AD  - Division of Clinical Laboratory, National Cancer Center Hospital, 5-1-1 Tsukiji, 
      Chuo-ku, Tokyo 104-0045, Japan.
FAU - Seki, Kunihiko
AU  - Seki K
FAU - Yokozawa, Karin
AU  - Yokozawa K
FAU - Tochigi, Naobumi
AU  - Tochigi N
FAU - Furuta, Koh
AU  - Furuta K
FAU - Hisaoka, Masanori
AU  - Hisaoka M
FAU - Hashimoto, Hiroshi
AU  - Hashimoto H
FAU - Shimoda, Tadakazu
AU  - Shimoda T
FAU - Hasegawa, Tadashi
AU  - Hasegawa T
LA  - eng
PT  - Journal Article
DEP - 20081124
PL  - England
TA  - Cancer Sci
JT  - Cancer science
JID - 101168776
RN  - 0 (DDIT3 protein, human)
RN  - 0 (EWS-FLI fusion protein)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Protein c-fli-1)
RN  - 0 (RNA-Binding Protein EWS)
RN  - 147336-12-7 (Transcription Factor CHOP)
SB  - IM
MH  - Aged
MH  - Female
MH  - *Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Liposarcoma/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Oncogene Proteins, Fusion/genetics
MH  - Proto-Oncogene Protein c-fli-1/genetics
MH  - RNA-Binding Protein EWS
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription Factor CHOP/*genetics
EDAT- 2008/11/29 09:00
MHDA- 2009/01/24 09:00
CRDT- 2008/11/29 09:00
PHST- 2008/11/29 09:00 [pubmed]
PHST- 2009/01/24 09:00 [medline]
PHST- 2008/11/29 09:00 [entrez]
AID - CAS1008 [pii]
AID - 10.1111/j.1349-7006.2008.01008.x [doi]
PST - ppublish
SO  - Cancer Sci. 2009 Jan;100(1):82-7. doi: 10.1111/j.1349-7006.2008.01008.x. Epub 
      2008 Nov 24.