PMID- 19043314
OWN - NLM
STAT- MEDLINE
DCOM- 20090116
LR  - 20211020
IS  - 1229-845X (Print)
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Linking)
VI  - 9
IP  - 4
DP  - 2008 Dec
TI  - Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed 
      with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs.
PG  - 387-93
AB  - This study was performed to evaluate the osteogenic effect of allogenic canine 
      umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) mixed with 
      beta-tricalcium phosphate (beta-TCP) in orthotopic implantation. Seven hundred 
      milligrams of beta-TCP mixed with 1 x 10(6) UCB-MSCs diluted with 0.5 ml of 
      saline (group CM) and mixed with the same volume of saline as control (group C) 
      were implanted into a 1.5 cm diaphyseal defect and wrapped with PLGC membrane in 
      the radius of Beagle dogs. Radiographs of the antebrachium were made after 
      surgery. The implants were harvested 12 weeks after implantation and specimens 
      were stained with H&E, toluidine blue and Villanueva-Goldner stains for 
      histological examination and histomorphometric analysis of new bone formation. 
      Additionally, UCB-MSCs were applied to a dog with non-union fracture. 
      Radiographically, continuity between implant and host bone was evident at only 
      one of six interfaces in group C by 12 weeks, but in three of six interfaces in 
      group CM. Radiolucency was found only near the bone end in group C at 12 weeks 
      after implantation, but in the entire graft in group CM. Histologically, bone 
      formation was observed around beta-TCP in longitudinal sections of implant in 
      both groups. Histomorphometric analysis revealed significantly increased new bone 
      formation in group CM at 12 weeks after implantation (p < 0.05). When applied to 
      the non-union fracture, fracture healing was identified by 6 weeks after 
      injection of UCB-MSCs. The present study indicates that a mixture of UCB-MSCs and 
      beta-TCP is a promising osteogenic material for repairing bone defects.
FAU - Jang, Byung Jun
AU  - Jang BJ
AD  - Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National 
      University, Seoul 151-742, Korea.
FAU - Byeon, Ye Eun
AU  - Byeon YE
FAU - Lim, Ji Hey
AU  - Lim JH
FAU - Ryu, Hak Hyun
AU  - Ryu HH
FAU - Kim, Wan Hee
AU  - Kim WH
FAU - Koyama, Yoshihisa
AU  - Koyama Y
FAU - Kikuchi, Masanori
AU  - Kikuchi M
FAU - Kang, Kyung Sun
AU  - Kang KS
FAU - Kweon, Oh Kyeong
AU  - Kweon OK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
RN  - 0 (Biocompatible Materials)
RN  - 0 (Bone Substitutes)
RN  - 0 (Calcium Phosphates)
RN  - 0 (beta-tricalcium phosphate)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/metabolism/therapeutic use
MH  - Bone Substitutes/*therapeutic use
MH  - Calcium Phosphates/*therapeutic use
MH  - Dogs
MH  - Fetal Blood/*cytology
MH  - Fracture Fixation/methods/veterinary
MH  - Mesenchymal Stem Cells/*physiology
MH  - Osteogenesis/*physiology
MH  - Tissue Engineering/methods
MH  - Wound Healing/physiology
PMC - PMC2811780
EDAT- 2008/12/02 09:00
MHDA- 2009/01/17 09:00
PMCR- 2008/12/01
CRDT- 2008/12/02 09:00
PHST- 2008/12/02 09:00 [pubmed]
PHST- 2009/01/17 09:00 [medline]
PHST- 2008/12/02 09:00 [entrez]
PHST- 2008/12/01 00:00 [pmc-release]
AID - 200812389 [pii]
AID - 10.4142/jvs.2008.9.4.387 [doi]
PST - ppublish
SO  - J Vet Sci. 2008 Dec;9(4):387-93. doi: 10.4142/jvs.2008.9.4.387.