PMID- 1904674
OWN - NLM
STAT- MEDLINE
DCOM- 19910715
LR  - 20151119
IS  - 0001-6772 (Print)
IS  - 0001-6772 (Linking)
VI  - 141
IP  - 2
DP  - 1991 Feb
TI  - Secretin dissipates red acridine orange fluorescence from pancreatic duct 
      epithelium.
PG  - 221-6
AB  - This study was undertaken to elucidate whether duct cells in the pancreas contain 
      acidic cytoplasmic compartments regulated by secretin. Microdissected pancreatic 
      ducts from pigs were examined by acridine orange (AO) and 
      2',7'-biscarboxyethyl-5(6)-carboxyfluorescein/tetraacetoxymethy l ester 
      (BCECF/AM) epifluorescence microscopy. Estimated cytoplasmic pH using BCECF 
      fluorescence was 7.43 +/- 0.04 and was not changed by altering CO2 tension in the 
      incubation medium. The epithelium of acridine orange incubated peripheral 
      interlobular pancreatic ducts exhibited green and red fluorescence; the colour 
      depending on the experimental conditions. Red epithelial fluorescence was seen in 
      resting pancreatic ducts and was greatly accentuated by raising CO2 in the 
      incubation medium from 5.5 to 10 kPa. The red fluorescence was abolished by 
      secretin, or following incubation with chloroquine or NH4Cl or the protonophores 
      carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) or carbonyl cyanide 
      m-chlorophenylhydrazone (CCCP), leaving uniform green fluorescence. These 
      findings suggest that pancreatic duct cells contain CO2-dependent acidic 
      compartments which vanish during secretin stimulation and which may be 
      cytoplasmic tubulovesicles.
FAU - Veel, T
AU  - Veel T
AD  - University of Oslo, Institute for Experimental Medical Research, Norway.
FAU - Buanes, T
AU  - Buanes T
FAU - Grotmol, T
AU  - Grotmol T
FAU - Ostensen, J
AU  - Ostensen J
FAU - Raeder, M G
AU  - Raeder MG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Acta Physiol Scand
JT  - Acta physiologica Scandinavica
JID - 0370362
RN  - 0 (Fluoresceins)
RN  - 01Q9PC255D (Ammonium Chloride)
RN  - 117464-70-7 (2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl 
      ester)
RN  - 1393-25-5 (Secretin)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 370-86-5 (Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone)
RN  - 555-60-2 (Carbonyl Cyanide m-Chlorophenyl Hydrazone)
RN  - 886U3H6UFF (Chloroquine)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*metabolism
MH  - Ammonium Chloride/pharmacology
MH  - Animals
MH  - Carbon Dioxide/pharmacology
MH  - Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology
MH  - Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone/pharmacology
MH  - Chloroquine/pharmacology
MH  - Cytoplasm/drug effects/metabolism/ultrastructure
MH  - Epithelium/drug effects/metabolism/ultrastructure
MH  - Fluoresceins
MH  - Hydrogen-Ion Concentration
MH  - Microscopy, Fluorescence
MH  - Pancreatic Ducts/drug effects/*metabolism/ultrastructure
MH  - Secretin/*metabolism
MH  - Swine
EDAT- 1991/02/01 00:00
MHDA- 1991/02/01 00:01
CRDT- 1991/02/01 00:00
PHST- 1991/02/01 00:00 [pubmed]
PHST- 1991/02/01 00:01 [medline]
PHST- 1991/02/01 00:00 [entrez]
AID - 10.1111/j.1748-1716.1991.tb09070.x [doi]
PST - ppublish
SO  - Acta Physiol Scand. 1991 Feb;141(2):221-6. doi: 
      10.1111/j.1748-1716.1991.tb09070.x.