PMID- 19047439
OWN - NLM
STAT- MEDLINE
DCOM- 20090210
LR  - 20211211
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 205
IP  - 13
DP  - 2008 Dec 22
TI  - Dendritic cells and B cells maximize mucosal Th1 memory response to herpes 
      simplex virus.
PG  - 3041-52
LID - 10.1084/jem.20082039 [doi]
AB  - Although the importance of cytotoxic T lymphocytes and neutralizing antibodies 
      for antiviral defense is well known, the antiviral mechanism of Th1 remains 
      unclear. We show that Th1 cells mediate noncytolytic antiviral protection 
      independent of direct lysis through local secretion of IFN-gamma after herpes 
      simplex virus (HSV) 2 infection. IFN-gamma acted on stromal cells, but not on 
      hematopoietic cells, to prevent further viral replication and spread throughout 
      the vaginal mucosa. Importantly, unlike other known Th1 defense mechanisms, this 
      effector function did not require recognition of virally infected cells via MHC 
      class II. Instead, recall Th1 response was elicited by MHC class II(+) 
      antigen-presenting cells at the site of infection. Dendritic cells (DCs) were not 
      required and only partially sufficient to induce a recall response from memory 
      Th1 cells. Importantly, DCs and B cells together contributed to restimulating 
      memory CD4 T cells to secrete IFN-gamma. In the absence of both DCs and B cells, 
      immunized mice rapidly succumbed to HSV-2 infection and death. Thus, these 
      results revealed a distinct mechanism by which memory Th1 cells mediate 
      noncytolytic IFN-gamma-dependent antiviral protection after recognition of 
      processed viral antigens by local DCs and B cells.
FAU - Iijima, Norifumi
AU  - Iijima N
AD  - Department of Immunobiology, Yale University School of Medicine, New Haven, CT 
      06520, USA.
FAU - Linehan, Melissa M
AU  - Linehan MM
FAU - Zamora, Melodie
AU  - Zamora M
FAU - Butkus, Debbie
AU  - Butkus D
FAU - Dunn, Robert
AU  - Dunn R
FAU - Kehry, Marilyn R
AU  - Kehry MR
FAU - Laufer, Terri M
AU  - Laufer TM
FAU - Iwasaki, Akiko
AU  - Iwasaki A
LA  - eng
GR  - AI064705/AI/NIAID NIH HHS/United States
GR  - AI054359/AI/NIAID NIH HHS/United States
GR  - R01 AI064705/AI/NIAID NIH HHS/United States
GR  - R01 AI054359/AI/NIAID NIH HHS/United States
GR  - R01 EB000487/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20081201
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigens, Viral)
RN  - 0 (Fas Ligand Protein)
RN  - 0 (Fasl protein, mouse)
RN  - 0 (fas Receptor)
RN  - 126465-35-8 (Perforin)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Antigens, Viral/immunology
MH  - B-Lymphocytes/*immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Dendritic Cells/*immunology
MH  - Fas Ligand Protein/immunology
MH  - Female
MH  - Herpesvirus 2, Human/*immunology/physiology
MH  - Humans
MH  - Immunologic Memory/*immunology
MH  - Interferon-gamma/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mucous Membrane/immunology
MH  - Perforin/genetics/immunology
MH  - Th1 Cells/*immunology
MH  - Vagina/cytology/immunology/virology
MH  - Virus Replication
MH  - fas Receptor/immunology
PMC - PMC2605233
EDAT- 2008/12/03 09:00
MHDA- 2009/02/12 09:00
PMCR- 2009/06/22
CRDT- 2008/12/03 09:00
PHST- 2008/12/03 09:00 [pubmed]
PHST- 2009/02/12 09:00 [medline]
PHST- 2008/12/03 09:00 [entrez]
PHST- 2009/06/22 00:00 [pmc-release]
AID - jem.20082039 [pii]
AID - 20082039 [pii]
AID - 10.1084/jem.20082039 [doi]
PST - ppublish
SO  - J Exp Med. 2008 Dec 22;205(13):3041-52. doi: 10.1084/jem.20082039. Epub 2008 Dec 
      1.